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甘蓝型油菜BnERF104超表达增强了转基因拟南芥对核盘菌的抗性 被引量：4

Over-expression of BnERF104 from Brassica napus enhanced transgenic Arabidopsis resistance to Sclerotinia sclerotiorum
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摘要 根据拟南芥乙烯响应因子AtERF104的序列设计保守引物,从甘蓝型油菜品种中双9号中克隆到同源基因BnERF104。序列分析表明BnERF104蛋白含有一个典型的ERF domain保守结构域,属于乙烯响应转录因子ERF家族的成员。甘蓝型油菜用核盘菌(Sclerotinia sclerotiorum)接种6h后,BnERF104基因被诱导显著上调表达。BnERF104基因在转基因拟南芥中的超表达提高了病原相关蛋白(PR)防御素PDF1.2和几丁质酶ChiB基因的表达量,显著增强了对腐生营养型真菌核盘菌的抗性。 BnERF104 gene was cloned from Brassica napus cv Zhongshuang 9 using conserved primers designed from AtERF104 gene sequence of Arabidopsis.NCBI-BLAST showed BnERF104 protein had a typical ERF conserved domain,was a member of ETHYLENE-RESPONSE-FACTOR（ERF） family.BnERF104 expression was up-regulated significantly at 6h after S.sclerotiorum inoculation.Over-expression of BnERF104 in transgenic Arabidopsis thaliana activated expressions of pathogenesis-related（PR） gene PDF1.2 and ChiB,and significantly enhanced plant resistance to necrotrophic fungi S.sclerotiorum.
作者 汪承刚 蔡丽 董彩华 黄军艳 赵美霞 周瑢 刘胜毅
机构地区 中国农业科学院油料作物研究所
出处 《中国油料作物学报》 CAS CSCD 北大核心 2011年第4期325-330,共6页 Chinese Journal of Oil Crop Sciences
基金 国家自然科学基金(30900934) 公益性行业(农业)科研专项(201103016)
关键词 乙烯响应转录因子 BnERF104 PR蛋白基因 核盘菌 ETHYLENE-RESPONSE-FACTOR BnERF104 PR gene Sclerotinia sclerotiorum
分类号 S565.4 [农业科学—作物学] Q78 [生物学—分子生物学]
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参考文献19

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二级参考文献13

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中国油料作物学报
2011年 第4期

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