摘要
目的:探讨中药大黄酸对大鼠慢性移植肾肾病(CAN)模型肾功能、组织学、转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)和纤维连接蛋白(FN)表达的影响。方法:F344近交系大鼠为供体、Lewis近交系大鼠为受体,左肾原位肾移植,建立30只CAN大鼠模型。将30只移植大鼠分成模型组(16只)和大黄酸治疗组(14只);在移植后1月、2月及4月分别收集大鼠的血、尿标本,检测肾功能及尿蛋白定量。移植后2月及4月分别宰杀大鼠,观察各组大鼠移植肾组织学变化及大黄酸对模型动物TGF-β1、CTGF和FN表达的影响。另取5只Lewis大鼠作对照组,行右肾切除,术后4月末行相应指标检测。结果:与模型组比较(1)大黄酸治疗组大鼠肾功能改善;肾组织学示肾间质纤维化及间质炎症明显改善;(2)大黄酸治疗组大鼠肾组织CTGF表达降低,细胞外基质FN明显降低。结论:大黄酸能改善CAN大鼠肾功能及肾组织慢性病变,降低CTGF表达,减轻肾脏纤维化。
Objective：To investigate the therapeutical effect of rhein in rats with chronic allograft nephropathy and to elucidate its mechanism. Methodology：We used Fisher rats as donors and Lewis rats as recipients to establish the model of chronic allograft nephropathy. Thirty rats with transplanting kidneys were divided into two groups： blank group for 16 rats and rhein intervention group with 14 rats. 5 lewis rats with right kidney removed were as controls. The blood and urine sample were collected at 4th, 8th and 16th week after transplantation for the examinations of renal function and total urine protein. The half of rats in each group was killed at 8th and 16th week for the renal histological examination. Immunohistochemical determination and real-time PCR were performed to detect the expression of transforming growth factor-β1 （ TGF-β1 ）, connective tissue growth factor（ CTGF）, fihronectin on renal tissue. Results ： （1） The rhein intervention group was significantly different from black group. The improved renal functions and significantly reduced the grade of renal fibrosis and interstitial inflammation were found after rhein intervention. （ 2 ） The expressions of CTGF as well as fibronectin were markedly decreased in renal tissue of rhein intervention group. Conclusion：The rhein can improve renal functions and reduce the grade of renal fibrosis and interstitial inflammation, which may associate with that rheln plays a role in antl-inflammation and anti-fibrosis.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
北大核心
2011年第4期342-345,351,共5页
Chinese Journal of Nephrology,Dialysis & Transplantation
关键词
大黄酸
慢性移植肾肾病间质纤维化
机制
rhein chronical allograft nephropathy renal fibrosis mechanis
