摘要
目的:摸索人脐静脉内皮细胞体外培养的方法和注意事项。方法:采用0.25%胰蛋白酶分离人脐静脉内皮细胞,用含20%FBS的DMEM培养基培养,待细胞80%融合后,以0.25%胰蛋白酶消化传代,并以倒置显微镜观察内皮细胞的生长情况,以免疫组化方法对内皮细胞进行鉴定。结果:原代培养的细胞在接种4h后开始贴壁生长,3～5天后融合成单层,倒置显微镜下观察细胞呈铺路石状排列,免疫组化显示人第Ⅷ因子相关抗原阳性。结论:用胰蛋白酶消化脐静脉可获得内皮细胞且成活率较高,是一种可靠的方法。
Objective ： To explore the culture and identification of the human umbilical vein endothelial cell （ HUVEC ） in vitro. Methods ： HUVECs were obtained from human umbilical vein by the technique of trypsinase digestion and cultivated in DMEM which containing 20% FBS; the passage was developed with 0.25% trypsinase when confluensing. The cells were identified under the microscope with immunohistochemical method. Results ： The primary cells began to attach and grow after being inoculated 4 hours, and grew to monolayer 3-5 days later. The antigens of human VIU factor were positive according to immunohistochemical method. Conclusion ： Trypsinase digestion is an effective way to cultivate the human umbilical vein endothelial cells in vitro.
出处
《辽宁中医药大学学报》
CAS
2011年第9期113-114,共2页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
人脐静脉
内皮细胞
培养
human umbilical vein
endothelial ceils
culture
