摘要
目的:探讨葡萄糖调节蛋白94(glucose-regulated protein94,GRP94)和CD8在宫颈病变组织中的表达及与HPV感染的关系。方法:采用免疫组化S-P法检测32例原发宫颈癌、27例宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及40例慢性宫颈炎组织中GRP94和CD8的表达及定位;采用western blot技术检测6例宫颈癌及6例正常宫颈组织中GRP94和CD8的表达。结果:①在宫颈癌、CIN2/3、CIN1及慢性宫颈炎组织中,GRP94的阳性表达率分别为87.5%、82.4%、40%和22.5%;CD8的阳性表达率分别为28.1%、64.7%、90%和97.5%;GRP94在宫颈癌和CIN2/3中的表达均显著高于慢性宫颈炎和CIN 1组织(P均<0.05);CD8在宫颈癌组的表达显著低于慢性宫颈炎组、CIN1组及CIN2/3组(P均<0.05)。②Western blot结果示GRP94在宫颈癌组织中的表达显著高于正常宫颈组织(P<0.01);CD8在宫颈癌组织中的表达显著低于正常宫颈组织(P<0.01)。③GRP94的表达与宫颈癌分化程度、有无脉管侵袭有关(P<0.05),而与年龄、病理类型、临床分期及有无淋巴结转移无关(P>0.05);CD8的表达与有无脉管侵袭有关(P<0.05),而与年龄、病理类型、临床分期、分化程度及有无淋巴结转移无关(P>0.05)。④宫颈病变组织中,GRP94的表达与HPV感染呈正相关(rs=0.377,P=0.000);CD8的表达与HPV感染呈负相关(rs=-0.395,P=0.000);GRP94与CD8的表达呈负相关(rs=-0.608,P=0.000)。结论:GRP94表达可能是宫颈CIN进展及宫颈癌预后判断的重要预测指标。
Objective： To investigate the expression of glucose-regulated protein 94 （GRP94） and CD8 in the pathological tissues of cervix and their relationship with HPV infection. Methods： S-P immunohistochemical staining was used to detect the expression and localization of GRP94 and CD8 in cervical tissues, which contained 32 cases of cervical cancer, 27 cases of cervical intraepithelial neo- plasia （C1N） and 40 cases of chronic cervicitis ; Western blot was used to detect the exprssion of GRP94 and CD8 in 6 cervical cancer and 6 normal cervical tissues. Results： ① In cervical cancer, CIN2/3, CIN1 and chronic cervicitis tissue the positite expression rates of GRP94 were 87.5%, 82.4%, 40% and 22.5%, respectively, while the positite expression rates of CD8 were 28.1%, 64.7%, 90% and 97.5%, respectively; The expression of GRP94 in cervical cancer and CIN2/3 were significantly higher than that in chronic cervicitis and CIN1 （P〈0.05）; The expression of CD8 in cervical cancer was significantly lower than that in chronic cervicitis, CIN1 and CIN2/3 （P〈0. 05）.② The expression level of GRP94 in cervical cancer was significantly higher than that in normal cervical tissue （P〈0.01）; The expression of CD8 in cervical cancer was significantly lower than that in normal cervical tissue （P〈0.01）.③ GRP94 protein expression had close relationship with differentiation and vessel invasion （P〈0.05）, but no relationship with age, clinical stage, histological type and lymph node metastasis （P〉0.05）. CD8 protein expression had close relationship with vessel invasion （P〈0.05）, but no relationship with age, clinical stage, histological type, differentiation and lymph node metastasis （P〉0.05）. ④ In cervical tissue, GRP94 expression was positively correlated with HPV infection （rs =0.377, P=0.000）; CD8 expression was negatively correlated with HPV infection （rs =-0.395, P=0.000）; GRP94 was negatively correlated with CD8 （rs =-0.608, P=0.000）. Conclusion： GRP94 may serve as potential predicters for progression of CIN and prognosis of cervical cancer.
出处
《现代生物医学进展》
CAS
2011年第17期3233-3239,共7页
Progress in Modern Biomedicine
基金
上海市科委基础研究重点项目(10JC1415600)
