摘要
目的观察Hedgehog信号通路相关蛋白Pteh、Smo和Glil在胰腺癌发生过程中的作用。方法200只SD大鼠分成对照组（20只）、二甲基苯并蒽（DMBA）组（90只）和环巴明治疗组（90只）。通过胰腺被膜下直接置人DMBA方法诱导胰腺癌模型。环巴明组在置入DMBA后每天腹腔注射6．25rnL／kg体重环巴明溶液。4个月后处死大鼠，观察胰腺组织病理学改变，应用免疫组化sP法检测胰腺癌及正常胰腺组织中Ptch、Smo、Glil的蛋白表达。结果DMBA组胰腺癌诱发成功率为57．5％（46／80），诱发的肿瘤最大径为0．5-〉2．0cm；环巴明组诱发成功率为17．1％（14／82），肿瘤最大径为0．5—2．0cm。两组胰腺癌发生率及肿瘤大小的差异具有统计学意义（P值均〈0．05）。DMBA组胰腺癌组织中Pteh、Smo及Glil阳性表达率分别为82．6％、73．9％和65．2％；环巴明组分别为50．0％、42．9％和28．6％，两组差异具有统计学意义（P值均〈0．05）。3组的正常胰腺组织中均无Ptch、Smo、Glil阳性表达。结论较大剂量DMBA置人胰实质内可在短期内获得较高的胰腺癌发生率；胰腺癌组织Hedgehog信号蛋白表达显著增加；环巴明能抑制Hedgehog信号蛋白表达，进而抑制胰腺癌发生和发展。
Objective To explore the expression and significance of hedgehog signal molecules （Ptch, Smo and Glil ） in pancreatic cancer. Methods Two hundred SD rats were randomly divided into DMBA group （ group A, n = 90 ） , cyclopamine intervening group （ group B, n = 90 ） and control group （ group C, n = 20 ）. For group A and B, DMBA was directly implanted into the parenchyma of the pancreas to establish the model of pancreatic cancer. The rats in group B were treated with 6.25 ml/kg cyclopamine and DMSO solution intraperitoneally daily. All rats were sacrificed four months later to observe the pancreatic tissue pathologic changes, and immunohistochemistry SP was used to detect the expression of Ptch, Smo, Glil protein in pancreatic cancer and normal pancreatic tissue. Results The prevalence rate of pancreatic cancer in group A was 57.5% （46/80）, the maximum size of the tumor was 0.5 - 〉 2 cm; the prevalence rate of pancreatic cancer in group B was 17.1% （ 14/82）, the maximum size of the tumor was 0.5 - 2.0 cm, and the difference between the two group was statistically significant （P 〈 0.05）. The positive expression rate of Ptch, Smo and Glil protein was 82.6%, 73.9% and 65.2% in DMBA group, and was 50.0%, 42.9% and 28.6% in cyclopamine group, and the difference between the two group was statistically significant （P 〈 0.05 ）. Ptch, Smo and Glil protein was expressed in normal pancreatic tissue. Conclusions Direct implantation of DMBA in the parenchyma of rat pancreas can induce pancreatic cancer with a high incidence in a short time.Hedgehog signal protein expression is significantly increased, cyclopamine can inhibit the occurrence and progression of pancreatic cancer by inhibiting Hedgehog messenger expression.
出处
《中华胰腺病杂志》
CAS
2011年第4期266-268,共3页
Chinese Journal of Pancreatology
基金
国家自然科学基金资助项目（30700360）
