摘要
目的观察亚硒酸钠对大鼠脑缺血再灌注损伤后行为学和梗死灶体积的影响。方法 63只SD大鼠随机分为假手术组、缺血再灌注组(模型组)及亚硒酸钠治疗组,每组21只,采用线栓法建立脑缺血再灌注模型。观察亚硒酸钠治疗前后大鼠行为学评分变化,于再灌注3d后亚甲兰染色观察海马CA1区神经细胞数量变化;进一步计算出脑组织含水量,TTC染色后测定脑梗死灶体积。结果模型组与亚硒酸钠治疗组治疗前大鼠行为学评分差异无统计学意义,亚硒酸钠治疗组与模型组比较,缺血再灌注后24h神经功能缺损即明显改善(P<0.05),72h神经功能改善更显著(P<0.01)。脑缺血再灌注损伤的大鼠海马神经细胞数目明显减少,亚硒酸钠治疗后海马神经细胞存活数目明显增多;治疗组与模型组相比,亚硒酸钠可以明显减少梗死灶体积(P<0.01)。与假手术组及非梗死侧大脑半球脑组织含水量比较,大鼠梗死侧脑组织含水量明显增加(P<0.05);与模型组比较,亚硒酸钠治疗后梗死区脑组织含水量明显降低(P<0.05)。结论亚硒酸钠能显著减轻大鼠脑梗死灶体积,从而改善神经功能症状,对脑缺血再灌注损伤有保护作用。
Objective To observe the effects of sodium selenite （Na2geO3） on the behavior and infarct size after cerebral ischemia-reperfusion （IR） injury in rats. Methods 63 Sprague-Dawley （SD） rats were randomly divided into three groups, the sham-operated group, IR group and the Na2SeO3 group, with 21 rats in each group. In the latter two groups, cerebral IR injury was induced by middle cerebral artery occlusion. The changes of behavior scores before and after the Na2SeO3 treatment in each group were studied, and the changes in the number of the hippocampal neu- rons by methylthionine staining three days after reperfusion was investigated. Then the cerebral water content was calculated and the volume of infarct was measured by TTC staining. Results There was no significant difference in the behavior scores before treatment between the IR group and the Na2SeO3 group, but neurological deficit was improved in the Na：SeO3 group 24 hours after reperfusion （P〈0.05）, and was significantly improved 72 hours after reperfusion （P〈0.01）. The number of hippocampal neurons significantly decreased after cerebral IR injury in rats, but the survival neurons increased significantly in the Na2SeO3 group after treatment. Compared with the IR group, the size of infarct was significantly reduced in the Na2SeO~ group （P〈0.01）. The brain tissue water content of infarcted cerebral hemisphere was increased significantly, compared with that of the sham-operated group and the non-infarcted cerebral hemisphere （,o〈0.05）. The brain tissue water content of the Na2SeO3 group was decreased significantly after treatment, compared with the IR group （P〈0.05）. Conclusion Na2SeO3 can reduce the infarct size and improve the neurological function in rats, thus exerting a protective effect on cerebral IR injury.
出处
《海南医学》
CAS
2011年第17期27-29,共3页
Hainan Medical Journal
关键词
亚硒酸钠
缺血再灌注损伤
脑梗死体积
大鼠
Sodium selenite （Na2SeO3）
Ischemia-reperfusion （IR） injury
Infarct size
Rat
