摘要
目的联合检测胃癌组织中端粒酶活性及基质金属蛋白酶(MMP2)蛋白的表达,旨在探索以上生物学指标与胃癌的生物学行为和患者预后的关系。方法应用TRAP-银染法、免疫组织化学Envision二步法分别检测40例胃癌组织中端粒酶活性、MMP2蛋白的表达。同时收集每一例胃癌患者的临床病理资料,并将上述标记物与患者的临床病理参数(年龄、性别、肿瘤大小、肿瘤部位、病理类型、组织分化程度、浸润深度、淋巴结转移情况、pTNM分期)进行相关性分析。结果端粒酶在正常胃粘膜的表达为13.3%(2/15),在胃癌组织中端粒酶阳性表达率为77.5%(31/40),差异显著(P<0.01);端粒酶的表达与肿瘤浸润深度、pTNM分期及有淋巴结转移呈正相关(P<0.01);MMP2在正常胃粘膜中呈阴性表达,胃癌组织中表达阳性率为44.35%,MMP2的表达强度与胃癌组织分化程度、淋巴结转移、浸润深度、pTNM分期呈显著相关(P<0.01)。Logistic多因素回归分析结果显示:端粒酶活性及MMP2蛋白的表达是与肿瘤浸润深度相关的危险因素(P<0.05);与pTNM分期相关的危险因素为MMP2蛋白的表达(P<0.05)。结论胃癌组织中端粒酶活性及MMP2蛋白的表达与胃癌的浸润转移密切相关,上述生物学指标的联合检测有助于对胃癌患者的转移和预后做出更准确的判断,更好地指导胃癌的治疗。
Objective To investigated the relationship between these biological markers and malignant behavior and prognosis in gastric cancer by combined detections of telomerase activity and MMP2 protein,Methods Telomerase activity and MMP2 protein expressions were determined in 40 gastric cancer samples（surgical resected specimen） using TRAP-sliver staining,immunohistochemistry staining（EnVision） respectively.The clinicopathological data of enrolled patients such as age,sex,tumor size,tumor site,pathologic type,histodifferentiation,infiltrating depth,lymph node metastasis and stage were obtained,and were made correlation analysis with these markers above.Results The telomerase activity in gastric cancer was significantly higher than in normal tissue（P0.01）,which was detected in 31（77.5%） of 40 gastric cancer tissues,and 2（13.3%） of 15 normal gastric tissues.The expression of telomerase was up-regulated with infiltrating depth,lymph node metastasis and stage（P〈0.01）.Positive expression rate of MMP2 in gastric cancer tissues was 44.35%,while negative expression in normal gastric tissues.The levels of MMP2 expression strongly correlated with histodifferentiation,infiltrating depth,lymph node metastasis and stage（P〈0.01）.In multivariate analysis of Logistic regression,telomerase activity and MMP2 proteins expression were hazardous makers that correlated with infiltrating depth（P〈0.05）,and MMP2 proteins correlated with stage（P〈0.05）.Conclusion Telomerase activity and MMP2 protein were closely related to infiltration and metastasis of gastric cancer,therefore combined detections of above biological makers could identify patients at high risk for disease recurrence and prognosis more efficiently.
出处
《贵州医药》
CAS
2011年第5期398-403,共6页
Guizhou Medical Journal
关键词
胃癌
端粒酶活性
MMP2
临床病理参数
预后
Gastric cancer Telomerase activity MMP2 Clinicopathological parametersPrognosis
