摘要
本研究旨在从分子水平上掌握中国新城疫病毒的变异情况和新城疫的流行规律,对2008-2009年从中国部分省市养殖场分离的9株新城疫病毒毒株,采用RT-PCR方法扩增其F和HN基因,经克隆和测序,对所得序列进行同源性和遗传进化分析。结果显示,9株分离株有8株为基因Ⅶ型,1株为基因Ⅱ型,F基因开放性阅读框架(ORF)为1662bp,强毒株同La Sota的核苷酸同源性为83.5%～84.2%。HN基因开放性阅读框架(ORF)为1716或1734bp,强毒株在538位缺失1个糖基化位点。结果表明,近年流行的ND疫情主要是由基因Ⅶ型NDV引起,F和HN基因的变异可能与频繁的疫苗免疫选择压力有关。
In order to explore the genetic mutations of the F gene and HN gene and the law of molecular epidemiology of Newcastle disease virus in China,nine influenza virus NDV were isolated from some poultry yards in China,the whole F and HN cDNA fragments of them were amplified by reverse transcription polymerase chain reaction,with primers specific to F gene and HN gene.After cloning and sequecing,gene sequences were analyzed on homology and heredity evolution.The sequence analysis showed 8 NDV isolates belonged to genotype Ⅶ,and 1 NDV isolates belonged to genotype Ⅱ.The F gene of NDV isolates had a 1662 bp open reading fragment（ORF） and the homology of the nucleotide of F gene is from 83.5% to 84.2%.The HN gene of NDV isolates had 1716 or 1734 bp.The glycosylation site of HN in virulent virus strains absence on 538 aa.The results revealed that Ⅶ genotype was the main genotype of NDV occurred in China now,variation of F gene and HN gene might be related to frequent vaccine selection pressure.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第8期85-89,共5页
China Animal Husbandry & Veterinary Medicine
关键词
新城疫病毒
F基因
HN基因
序列分析
基因型
Newcastle disease virus（NDV）
F gene
HN gene
sequence analysis
genotype
