摘要
通过RT-PCR方法扩增到犬瘟热病毒(canine distemper virus,CDV)分离株GS0812-4的N、H、F蛋白基因,然后克隆入pGEM-T Easy载体并进行序列分析和抗原表位预测分析。结果表明,GS0812-4分离株的N、H、F蛋白基因与其他分离株相应序列的核苷酸同源性分别为93.2%～97.8%、90.3%～97.3%、93.2%～97.8%,其推定的氨基酸同源性分别为96.6%～98.7%、91.3%～97.9%和96.6%～98.7%;GS0812-4分离株N、H、F蛋白基因与疫苗株相应序列的核苷酸同源性分别为93.1%～93.8%、89.4%～90.5%、93.1%～93.8%,其推定的氨基酸同源性分别为96.4%～97.3%、89.3%～91.3%、96.4%～97.3%。由基因系统发育树可知,GS0812-4与疫苗株处于不同的分支,其中与MKY-KM08的亲缘关系最近,但GS0812-4在N、H、F 3个基因系统发育树上与其他同一毒株的亲缘关系远近不同。H、F蛋白的抗原表位预测结果表明,GS0812-4与MKY-KM08和疫苗株的抗原表位均有差异。说明GS0812-4是野毒株,与MKY-KM08属于同一基因型,来源于同一毒株,但两毒株间的H蛋白和F蛋白的抗原表位仍有差异。
The nucleoprotein（N）,haemgagglutinin protein（H）and fusion protein（F） genes of canine distemper virus（CDV） isolate GS0812-4were obtained by RT-PCR and sequenced after being cloned directly into the pGEM-T Easy.Thesequences of N,H and F genes of GS0812-4 were compared with sequences and antigen epitope of other CDV strains in GenBank by BLAST and DNAStar software.The results showed that the N,H and F of GS0812-4 shared 93.2% to 97.8%,90.3% to 97.3%,93.2% to 97.8% of nucleotide homology,and 96.6% to 98.7%,91.3% to 97.9% and 96.6% to 98.7% of amino acid identity with that of referenced strains,respectively.However,The N,H and F genes of the GS0812-4 shared only 93.1% to 93.8%,89.4% to 90.5% and 93.1% to 93.8% of nucleotide homology,and 96.4% to 97.3%,89.3% to 91.3% and 96.4% to 97.3% of amino acid homology with that of vaccine strains,respectively.Phylogenetic tree showed that GS0812-4 was different from the vaccine strain of the branch,it had a recent genetic relationship and MKY-KM08.But GS0812-4 compared with other strains of different genetic distance relationships.H,F protein epitope prediction results showed that GS0812-4 epitope was different from and MKY-KM08 and vaccine strains.Results showed that GS0812-4 was the wild strain,and the GS0812-4 strain was MKY-KM08 belonged to the same genotype,derived from the same strain,but there were still differences between epitopes.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第8期71-77,共7页
China Animal Husbandry & Veterinary Medicine
基金
中央级公益性科研院所基本科研业务费专项资金项目(BRF080302)
关键词
犬瘟热病毒
H蛋白
H蛋白
F蛋白
变异分析
canine distemper virus（CDV）
nucleoprotein（N）
fusion protein（F）
haemgagglutinin protein（H）
genetic diversity
