摘要
根据基因库中新城疫病毒(NDV)的NP基因序列设计了1对特异性引物,应用RT-PCR技术对广西在2000～2003年暴发新城疫的鸡群中分离的11株NDV毒株NP基因进行RT-PCR扩增和序列测定,拼接出11个NDV广西分离株的NP基因的全序列,10个NDV广西分离株的NP基因阅读框的核苷酸序列全长均为1470 bp,编码489个氨基酸,它们的NP基因核苷酸全序列及推导的氨基酸全序列与10个已发表的NDV参考株的NP基因全序列比较分析结果表明:核苷酸序列同源性为84.8%～98.2%,氨基酸同源性为89.8%～99.4%.
One pair of specific primers were designed and synthesized according NP gene sequences dates of Newcastle Disease Virus （NDV） strains from GenBank. NP genes of eleven NDV isolates from Guangxi current outbreak ND chicken farms between 2000-2003 were amplified by reverse transcription-polymerase chain reaction（RT-PCR） technique. The NP genes were sequenced after the products of amplification being cloned, and then we got the whole sequences of NP genes of eleven NDV isolates in Guangxi. All nucleotide sequences of NP gone open read fragment of eleven NDV isolates in Guangxi are 1470 bp encoding 489 amino acids. Comparison of their NP protein sequences with 10 other published NDV NP gene sequences reveals that the homologies of the nucleotide arebetween 84.8 %-98.2 %, and the homologies of the deduced amino acid sequences are between 89.8 %-99.4 %.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2005年第5期336-339,343,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
广西科技攻关项目(桂科攻0235001-4)
广西留学基金项目(桂科回0236005)
广西水产畜牧局科研计划资助
关键词
新城疫病毒
F蛋白基因
克隆
序列分析
newcastle disease virus
NP gone
cloning
sequence analyses
