摘要
用荧光测量方法，对被认为可作本底水样的几种水体进行了荧光特性的测量研究，确认了即时处理的Milli-Q水是无荧光效应的高纯度水，可作为本研究的本底水样。同时，对海水黄色物质Milli-Q水溶液、市售草炭腐殖质Milli－Q水溶液、青岛近岸海水和褐藻培养液样品进行了光谱特性测量研究。结果表明，海水黄色物质和培养液的黄色物质的激发光谱峰值波长在340nm附近、荧光光谱峰值波长在435nm附近；而草炭腐殖质的激发峰值波长在320nm附近，荧光峰值波长在440nm附近。测量得出了海水黄色物质Milli-Q水溶液的荧光强度与其浓度的关系曲线。其最低检测浓度高达1×10－9／ml以上。同时也测量得出了青岛近岸海水样品不同稀释度的稀释液与对应的荧光强度的良好正相关关系。用荧光法测量得出青岛近岸海水的黄色物质浓度为3．2×10－7g／ml。
The inherent seawater fluorescence is produced by yellow substance in seawater.Therefore, study on the fluorescence cbeteristics of yellow substance is important, because seawater yellow substance plays an importan role in som marine procedures.Based up physical analysis, we study the yellow subetance fluorescence character in order to measare the concentation,distribution and optical property of yellow substance and to identify its appication to marine process studies.The fluorescence character of some water samples as blank was measured and studied; the free-fluorescence high pure Milli-Q warer was selected in the experiment as a boe-ine water- Then,spectra of the sampes, such as yellow substance solution in Milli-Q water- Qngdao coastal water and algae filtrate (passing through a 0.45μm filter) were analysed. The results show tha seawater yellow substance has sam fluorescence peak near 435nm for 340nm excitation as algae filtrate but 440nm emission peak at 320nm excitation for peat hdric subeate.The concentration of yellow subetance solution in Milli-Q water Vs fluorescence relative intensity plot (standard curves) is asqinred, with the lowest detechng concentration of 1×10-9/ ml.The relationship betWeen the various dilution fastors of solution and the Qngdao coastal water and fluorescence relative intensity shows an excellent linear cormlation- The measared yellow substance concemtiaon in the Qngdao coastal water is 3.2×10-9/ml.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1999年第6期719-725,共7页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金!49786005
中丹国际合作项目![97]国科金外字054号
关键词
海水黄色物质
荧光特性
激发光谱
荧光光谱
Yellow substance in seawater Fluorescence character Excitation spectrum Fluorescence spectrum
