摘要
目的应用核酸扩增技术对母婴传播婴幼儿人类免疫缺陷病毒（HIV）感染进行早期诊断,为临床应用提供依据。方法对2009-2010年HIV阳性孕产妇活产的34例3-14个月龄婴幼儿收集足底干血斑样本进行HIV-1 DNA检测。随访18个月后进行血清HIV抗体检测比较。结果 34例婴幼儿中,检出HIV-1 DNA 9例,检出率26.5%。9例HIV-1 DNA阳性患儿均未使用过抗病毒治疗。18个月后随访,34例婴幼儿除1例失访,2例检出HIV-1 DNA婴儿已死亡（根据临床有机会性感染体征诊断为AIDS）,7例检出HIV-1 DNA婴儿血清抗体阳性外,其他24例未检出HIV-1 DNA的婴儿其血清抗体为阴性。两种方法检测结果比较符合率为100.0%。结论核酸检测是有效的早期诊断方法,早期诊断有利于后续开展母婴阻断工作。
Objective To establish and evaluate polymerase chain reaction for the early detection of HIV type 1 in infants.Methods A total of 34 dried blood spot（DBS） samples were collected during 2009 and 2010 from 3-14 months infants of HIV positive mothers.All the specimens were tested by qualitative HIV-1 DNA polymerase chain reaction.HIV antibodies were tested with Western blot assay after 18 months following-up.Results HIV-1 DNA was positive in 9（26.5%） samples by HIV-1 DNA polymerase chain reaction.Of 9 HIV-1 DNA positive infants,7 were HIV antibodies positive and 2 died of AIDS related opportunity infection after 18 months follow-up.There was no anti-HIV positive conversion among 25 HIV-1 DNA negative infants.Conclusions HIV-1 DNA polymerase chain reaction is a sensitive assay for the early detection of HIV-1 infection among newborns and infants.It should be helpful for the prevention of mother-to-child transmission of HIV.
出处
《中国病毒病杂志》
CAS
2011年第4期279-281,共3页
Chinese Journal of Viral Diseases
基金
全球基金中国艾滋病防治项目(CHN-304-G03-H)
湖南省卫生厅科研计划项目(B2010-091)
关键词
母婴传播
HIV-1DNA检测
蛋白印迹试验
HIV抗体
干血斑样本
Mother-to-child transmission
HIV-1 DNA polymerase chain reaction
Western blot test
HIV antibody
Dried blood spot（DBS） sample
