摘要
本实验以红细胞膜带Ⅲ蛋白Ｃ１（Ａｌａ Ｓ９３－Ｖａｌ９１１）及ＫＳ－２（Ｓｅｒ ７３１－Ｌｙｓ ７４３）／肽链的释放量为指标，研究了不同浓度的氢氧化钠对带Ⅲ蛋白的影响。结果证明随着氢氧化钠浓度的升高多肽的释放量明显增多，用１００ｍ Ｍ ＮａＯＨ 预处理红细胞膜，再用胰蛋白酶消化，对从红细胞膜释放的多肽进行了氨基酸测序分析。
We used the C1 (Ala 893 Val 911) and KS 2(Ser 731 Lys 743) peptides of band 3 protein as indicators to study the effect of NaOH on the band 3. Five peptides were released from the band 3 molecule when 100mM NaOH stripped membrane was digested with trypsin. All of the five peptides were sequenced.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
1999年第4期435-437,共3页
Journal of Biomedical Engineering
