摘要
目的 :从人脑垂体中获得纯化催乳素 ( PRL) ,生长激素 ( GH) ,促卵泡激素 ( FSH) ,促黄体生成激素 ( LH)和促甲状腺激素 ( TSH) ,应用于医学实验研究。方法 :以冷冻人脑垂体为原料经离子交换层析、凝胶过滤和亲和层析等方法可获得达国际标准品水平的激素纯品。结果 :最高放射免疫活性分别可达 PRL36.2 /k IU/g,FSH3333k IU/g,LH1 5842 k IU/g,TSH1 1 .49k IU/g,GH2 .42 k IU/g。结论 :利用此方法提取与纯化人脑垂体激素简便。
Objective:There are glycoprotein hormones and peptides in human pituitary glands such as prolactin(PRL)、Follocle stimulation hormone(FSH)、Thyroid stimulating hormone(TSH)、Luteinizing hormone(LH) and Growth hormone (GH) and using these hormones for medical test. Methods:These hormones were extracted and purified from cooled human pituitary glands by affinity chromatography、gel filtration chromatography and so on .Results:The radiommunoassay potency of pure hormones arrived at international standard level such as PRL 36.27kIU/g、FSH3333kIU/g、LH15842kIU/g、TSH11.49kIU/g、GH2.42kIU/g.Conclusion:These pure hormones which extract and purify from human pituitary glands are used in laboratory for example RIA、ELISA、production hormone KIT and production antibody by immunization animal.
出处
《天津医科大学学报》
1999年第4期26-27,共2页
Journal of Tianjin Medical University
关键词
人脑垂体
促激素
纯化
human pituitary glands
trophic hormone
purification
