摘要
报道了用拉曼光谱法测定紫外线诱变脱氧核糖核酸（ＤＮＡ）前后的拉曼散射结果。实验结果表明，经过紫外线诱变以后，Ａ－ ＤＮＡ型结构特征谱线８１０ｃｍ －１ 减弱；分别反应脱氧核糖磷酸盐链振动和Ｃ－ Ｏ 伸缩振动的谱带８８４ｃｍ －１ 和１０１０ｃｍ －１ 、１０６８ｃｍ －１ 都有不同程度的减弱；属于脱氧核糖在１４６８ｃｍ －１ 处的谱线显著减弱；还有个别碱基谱带出现（在６７０ｃｍ －１ 处的胸腺嘧啶Ｔ） 、增强（ 在１３０４ｃｍ －１ 处的腺嘌呤Ａ）和消失（在６５２ｃｍ －１ 处的胸腺嘧啶Ｔ）、减弱（ 在１５２６ｃｍ －１ 处的鸟嘌呤Ｇ和胸嘧啶Ｃ）。
The results of Raman scattering of solid deoxyribonucleic acid(DNA) obtained at the fore and aft induced by ultraviolet radiation by Raman spectroscopy are reported.the experimental results show that structural marker line of A DNA conformation at 810cm -1 decreases,the Raman bands assigned respectively to deoxyribose phosphate and C O stretch at 884cm -1 and 1010cm -1 、1068cm -1 all decrease differently degree,one Raman peak from deoxyribose at 1468cm -1 decreases markedly,the marker band of some base appears(thymine at 670cm -1 )、increases(adenine at 1304cm -1 )and disappears(thymine at 652cm -1 )、decreases(guanine and sytosine at 1526cm -1 ),after DNA is induced by ultraviolet radiation.
出处
《激光杂志》
CAS
CSCD
北大核心
1999年第6期12-13,共2页
Laser Journal
基金
河南省教委科研基金
关键词
紫外线
诱变
DNA
拉曼光谱
ultraviolet radiation,induce,DNA,Raman spectroscopy
