摘要
目的确定80例口腔颌面部感染的细菌类型，研究厌氧菌在其中的分布及厌氧菌代谢产物在其鉴定中的辅助作用。方法对脓液标本进行分离培养，同时应用GLC技术分析厌氧菌代谢产物辅助确定厌氧菌种、属。结果80例口腔颌面部感染的脓液标本全部分离出细菌。厌氧菌检出率95.00％，分布于8个属、29个种，检出率高的厌氧菌依次为消化链球菌、消化球菌、类杆菌、按形杆菌、韦荣氏菌、真杆菌等。厌氧菌与需氧菌混合感染率为80.00％，需氧菌与厌氧菌的比例为1：1.08。结论口腔颌面部感染大多数是以厌氧菌为优势菌的内源性混合感染。应用高效、高敏、高选择性、高分辨力的GLC技术分析厌氧菌代谢产物，对确定厌氧菌种、属有决定性意义，是目前较有前途并值得推荐的快速厌氧菌诊断方法。
Objective To define the infection types of 80 cases of ora-maxillofacial infection and investigate its anaerobes' distribution and the role of the anaerobes' metabolic products in their identification. Methods We isolated and cultured anaerobes from the purulent specimen and applied the gasliquid chromatograph (GLC) technique to analyze the anaerobes' metabolic products to define their genera and species in the meantime. Results Bacteria were isolated from all of the purulent specimen.lsolation rate of bacteria was 100. 00% and isolation rate of anaerobes, which distributed in 8 genera, 29 species .was 95.00%. The higher detectable rate of them were respectively peptostreptococcus. petococcus,bacteroides. fusobacterium, verillonella, eubacterium. The rate of mixed infection was 80. 00%. The proportion ratio of aerobes and anaerobes was 1: 1. 08. Conclusion Oral-maxillofacial infection mostly belongs to endogenous mixed infection in which anaerobes are preponderant bacteria. GLC technique is a effective,sensitive,selective and specific method for defining anaerobes' genera and species fhrough analyzing their metabolic products. It is a promising and recommendable rapid anaerobes' identification method comparatively at present.
出处
《上海口腔医学》
CAS
CSCD
1999年第4期213-216,共4页
Shanghai Journal of Stomatology
关键词
口腔颌面部感染
厌氧菌
分离培养
GLC技术
ora-maxillofacial infection
anaerobes
isolation and culture of the anaerobes
GLC technique
