摘要
目的:探讨凋亡相关蛋白Survivin(生存素)和Caspase-3(半胱氨酸蛋白酶-3)在子痫前期患者与正常妊娠妇女胎盘组织中的表达规律及其在子痫前期发病过程中可能的机制。方法:随机选取子痫前期患者60例(轻度组25例,重度组35例)和正常妊娠孕妇30例作为病例组和对照组,应用免疫组织化学(SABC)法对3组胎盘的Survivin、Caspase-3进行标记,通过彩色病理图像分析系统对染色结果进行定量检测并分析。结果:①Survivin在子痫前期重度组表达低于轻度组及对照组,差异有统计学意义(P<0.01);轻度组与对照组比较,差异无统计学意义(P>0.05);②Caspase-3在子痫前期重度组表达高于轻度组及对照组,轻度组表达高于对照组,差异均有统计学意义(P<0.01);③Survivin与Caspase-3在3组胎盘组织中表达呈负相关(r=-0.370,P<0.05)。结论:子痫前期患者胎盘组织中凋亡相关蛋白Survivin表达下调、Caspase-3表达上调,Survivin及Caspase-3的表达变化可能与子痫前期的发病机制有关。
Objective:To investigate the expression of Survivin and Caspase-3 and association between them in placenta tissue of preeclampsia,and to explore the etiology and mechanism of preeclampsia.Methods:60 placentas tissues samples were abstained from preeclampsia patients including 25 mild patients and 35 severe patients and 30 normal placentas tissues were enrolled from the third trimester pregnancies.Immunohistochemistry(SABC) method was used to study the expression of Survivin and Caspase-3 in placenta tissue.Furthermore,multi-function precise color-image measure system was used for quantitative analysis.Results:① The expression of Survivin in severe group was lower than that in mild group and normal group(P<0.01).The expression in mild group was not lower than that in normal group(P>0.05).② The expression of Caspase-3 in severe group was higher than that in mild group and normal group(P<0.01).The expression in mild group was higher than that in normal group(P<0.01).③Negative relationship between the expression of Survivin and Caspase-3 could be found with statistical significance(R=-0.370,P<0.05).Conclusion:Lower expression of Survivin and higher expression of Caspase-3 may play an important role in the pathogenesis of preeclampsia.The imbalance between Survivin and Caspase-3 expression may induce preeclampsia.
出处
《中国妇幼保健》
CAS
北大核心
2011年第20期3148-3150,共3页
Maternal and Child Health Care of China
