摘要
应用逆转录－多聚酶链式反应（ＲＴ－ＰＣＲ）技术，检测了３５ 例喉癌患者ＭＤＲ－１ ｍ ＲＮＡ、ＭＲＰｍ ＲＮＡ 基因的表达，同时，应用免疫组化ＳＡＢＣ法检测了６１ 例患者ＭＤＲ－１ 基因产物Ｐ－ｇｐ。喉癌组织中ＭＤＲ－１ ｍ ＲＮＡ 及Ｐ－ｇｐ 的阳性表达率分别为３７．１％ （１３／３５）和３４．４％ （２１／６１）；３５ 例ＲＴ－ＰＣＲ 检测标本中，两项指标间有一定的相关性（Ｐ＜ ０．０１）。晚期患者（Ｔ３－ ４，１８／４０）Ｐ－ｇｐ 阳性表达率明显高于早期患者（Ｔ１－ ２，３／２１，Ｐ＜ ０．０５）。喉癌组织中ＭＲＰｍ ＲＮＡ阳性表达率为４５．７％ （１６／３５），其阳性表达强度在晚期肿瘤中（１４／２３）明显增高（Ｐ＜ ０．０５），且与肿瘤颈淋巴结转移（Ｎ０，８／２７；Ｎ１－ ２，８／８）呈明显相关性（Ｐ＜ ０．０１）
Reverse transcription polymerase chain reaction (RT PCR) assay was used to detect the expression of MDR 1 mRNA and MRP mRNA in 35 patients. Meantime, Immunohistochemistry SABC technique was used to detect P glycoprotein(P gp) in 61 cases. The positive expression rate of MDR 1 mRNA and P gp in laryngeal carcinoma were 37.1 percent(13/35) and 34.4 percent(21/61), respectively. There was significant relationship between MDR 1 mRNA and P gp(P<0.01) in 35 cases of RT PCR assay. The positive expression rate of P gp in advanced(T 3 4 ) patients was higher than that in early(T 1 2 ) cases (P<0.05). The positive expression rate of MRP mRNA was 45.7 percent (16/35). It was higher in advanced patients than that in early eases(P<0.05) and showed a significant correlation with metastases of cervical lymph node(P<0.01).
出处
《山东医科大学学报》
1999年第4期333-336,共4页
Acta Academiae Medicinae Shandong
关键词
喉肿瘤
多药抗药性
基因表达
RT-PCR
Laryngeal neoplasms
Drug resistance, multiple
Gene expression
Reverse transcription polymerase chain reaction (RT PCR)
Immunohistochemistry
