摘要
背景:研究已经证实海藻糖对低温保存中的胸骨具有保护作用,但作用途径尚不清楚。目的:观察海藻糖对低温保存胸骨bcl-2和bax基因表达的影响。方法:新鲜配置4组保存液:低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组、低钾右旋糖酐+海藻糖组、低钾右旋糖酐+二甲基亚砜组+海藻糖组。切取SD大鼠胸骨后立即分别放入含上述4种溶液的冻存管中低温保存。抽取新鲜大鼠胸骨组织和低温保存120d的4组标本,采用RT-PCR方法检测不同保存液保存的胸骨及新鲜胸骨bcl-2和bax基因mRNA表达量。结果与结论:低钾右旋糖酐+海藻糖组bcl-2表达量高于低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组(P<0.01),但bax表达量低于低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组(P<0.01);低钾右旋糖酐+二甲基亚砜组+海藻糖组bcl-2表达量最高,bax表达量最低,基本接近新鲜骨组织(P>0.05)。结果提示海藻糖可能通过增强bcl-2基因、抑制bax基因的表达保护低温保存中的胸骨细胞活性,其与二甲基亚砜合用保护作用更好。
BACKGROUND：Studies have demonstrated that trehalose possesses protective effects on cyropreserved sternum.But the mechanism of action remains poorly understood.OBJECTIVE：To investigate the effects of trehalose on bcl-2 and bax mRNA expression in cryopreserved sternum.METHODS：Four groups of freshly prepared solution were used：low-potassium dextran（LPD）,LPD＋dimethyl sulfoxide（DMSO）,LPD＋trehalose,LPD＋DMSO＋trehalose.Rat sternum was cut and then immediately cryopreserved in the tubes containing each group of solution.Fresh rat sternum tissue and 4 groups of samples cryopreserved for 120 days were taken and bcl-2 and bax mRNA expression in fresh and cryopreserved sternum was detected using reverse transcription-polymerase chain reaction.RESULTS AND CONCLUSION：bcl-2 mRNA expression in the LPD＋trehalose group was significantly higher,but bax mRNA expression was significantly lower,than in the LPD,LPD＋DMSO groups（both P0.01）.LPD＋DMSO＋trehalose group showed highest bcl-2 mRNA expression and lowest bax mRNA expression,which were basically similar to fresh bone tissue（P0.05）.These findings indicate that trehalose may protect cell activity in cryopreserved sternum by enhancing bcl-2 mRNA expression and inhibiting bax mRNA expression,and trehalose together with DMSO shows better protective effects.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第21期3975-3978,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
a grant from Science and Technology Bureau of Qingdao City,No.06-2-2-6-nsh-2~~
