摘要
将锦鲫(Carassius auratus)幼鱼20尾随机平均分成训练组和对照组,在25℃水温下每天饱足投喂1次;其中训练组实验鱼持续2周进行强度为每天2次的力竭运动训练;随后分别测量实验鱼力竭运动后的耗氧率并计算过量耗氧(EPOC);待力竭运动恢复8 h后再分别取样测量肌乳酸、肝乳酸的含量和肌肉中乳酸脱氢酶(LDH)、柠檬酸合成酶(CS)的活性。研究发现,力竭运动训练组与对照组的EPOC分别为(166.2±7.2)、(108.0±6.2)mg.kg-1,训练组显著高于对照组(p<0.05);肌乳酸含量分别是(17.4±0.8)、(25.4±1.7)μmol.g-1,训练组显著低于对照组(p<0.05);训练组实验鱼的肝乳酸含量、LDH和CS的活性与对照组之间均无显著差异。研究表明力竭运动训练有助于提高锦鲫幼鱼的无氧代谢能力;无氧代谢能力的提高可能与乳酸清除速率加快有关。
Twenty juvenile crueian carp were set to two groups （exercise trained group and control group ） , and all fish were fed once daily at 25℃. Exercise-acclimated fish underwent two weeks exhaustive chasing training twice daily. Then the excess post-exercise oxygen consumption （EPOC） was measured after a bout of exhaustive chasing in both the exercise-acclimated group and control-acclimated group. The lactic acid content in muscle and liver, the activity of lactate dehydrogenase （LDH） and citrate synthase （CS） in muscle were also measured 8 hours ＇after exercise. The results revealed that the EPOC magnitude of the exercise-trained group（166.2±7.2） was significantly larger than that of the control group（108.0±6.2）mg·kg^-1while the content of muscle lactic acid in the exercise-trained group （17.4 ± 0.8）μmol· g^-1 was significantly lower than that in the control group （25.4 ±1.7）μmol · g^-1 （p 〈 0.05）. Exhaustive chasing training had no significant effect on the either content of liver lactic acid or activities of muscle LDH and CS. The results suggested that exhaustive chasing training can significantly enhance the anaerobic metabolic eapacity for juvenile crucian carp and the elevation of the anaerobic metabolic capacity may be associated with the enhanced clearance rate of lactic acid.
出处
《重庆师范大学学报（自然科学版）》
CAS
2011年第4期16-18,22,共4页
Journal of Chongqing Normal University:Natural Science
基金
国家自然科学基金(No.30700087)
重庆市高校优秀人才支持计划(2009)
重庆市自然科学基金(No.CSTC2010BB1089)
关键词
力竭运动训练
过量耗氧
乳酸
锦鲫
exhaustive exercise training
excess oxygen consumption
lactic acid
Carassius auratus
