摘要
为优化酶解卵白蛋白制备抗氧化肽的工艺条件。以酶解物羟自由基(HO.)清除率为指标,在单因素实验的基础上选取实验因素和水平,根据Box-Behnken设计原理采用三因素三水平的响应面分析法,研究底物浓度,酶添加量和水解时间工艺的影响,对酶解条件进行优化研究。结果胃蛋白酶酶解卵白蛋白制备抗氧化肽的最优条件为:底物浓度3.24%、胃蛋白酶添加量11 332.12 U/g蛋白、水解时间7.8 h。酶解液的羟自由基清除率为44.20%,与预测值的相对误差为4.088%,理论值与预测值基本一致,与模型预测具有较好的拟合性。结论建立的模型在实践中进行预测是可行的。
In order to optimize enzymolysis conditions for production of antioxidant peptides from egg ovalbumin.Hydroxyl radical scavenging activity was chosen as the response value,on the basis of single factor test,combined with central composite experimental design principle,response surface methodology was used to optimize the technical parameters.The optimum hydrolytic conditions were substrate concentration 3.24% with enzyme concentration of 11 332.12 U/g pro and enzymolysis time of 7.8 h.The maximum scavenging rate of hydroxyl radical was 44.2%.The relative error was 4.088%,and the actual experimental value was basically consistent with the predicted value of the mode1.The predicted model could reflect actual circumstances well.Their predictive models are feasible in practice.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2011年第3期589-596,607,共9页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省教育厅项目(GJJ98134)
关键词
响应曲面法
卵白蛋白
酶解
抗氧化肽
response surface methodology
ovalbumin
antioxidant peptides
enzymatic hydrolysis
