摘要
菊芋富含菊粉，菊粉经菊粉酶一步酶解，可生产高果糖浆．报道了菊芋发酵生产菊粉酶、高果糖浆、酒精等研究结果，国内外研究进展以及酶解产物的综合利用．经诱变选育出的高产突变株ＡＬ１５４ 菊粉酶的活力达１４６ ．３ μ／ｍＬ，比出发株提高近３ 倍，菊粉酶酶解菊芋提取液的最适工艺条件为：ｐＨ５ ．０ ，６０ ℃，底物总糖浓度１０％ ～２０ ％ ，酶用量３ ．０ μ／ｇ菊糖，酶解时间１０ ｈ ，底物降解率达９７ ．２ ％ ，酶解产物中果糖占总糖的８６ ．１ ％ ．
Jerusalem artichoke is rich in Inulin . Directly decomposig Inulin with Inulalse can produce high fructose syrup. This article mainly introduces the research achievement, comprehensive utilization of fermented products, preparation of Inulalse , high fructose syrup and alcohol by using microbial fermentation process and pointed out the prospect of fermented products in food industry. A mutant AL 154 Inulase activity reaches 146.3 μ/mL. The suitable conditions of enzymolysis are as follows: Jerusalem artichoke extracted juice with 10%～20% of the total suger as the substrate, 3.0 units (the activity unit using Inulin as substrate) of enzymatic activity used pergram total sugar of the substrate, pH5.0, temperature 60℃, with enzymolysis for 10h. Under these conditions, the highest hydrolytic rate of the substrate is 97.2%. The hydrolysate containes about 86.1% fructose.
出处
《四川师范大学学报（自然科学版）》
CAS
CSCD
1999年第6期747-751,共5页
Journal of Sichuan Normal University（Natural Science）
基金
四川省教委青年科研基金
