摘要
目的和方法：为进一步阐明肿瘤血路转移的分子机制，采用活性染料玫瑰红摄入法，研究人卵巢癌细胞系ＨＯ－ ８９１０ 与激活的人脐静脉内皮细胞（ ＨＵＶＥＣｓ） 的粘附性以及抗粘附分子Ｅ－ 选择素（ Ｅ－ ｓｅｌｅｃｔｉｎ） 及其配体ｓＬｅｘ 单抗对ＨＯ－ ８９１０ 细胞与激活的ＨＵＶＥＣｓ 粘附的影响。结果：ＨＯ－ ８９１０ 细胞与血管内皮细胞的粘附能力明显高于低转性的人大肠癌ＨＲ－ ８３４８ 细胞。当血管内皮细胞被ＬＰＳ（１ μｇ／ｍ Ｌ） 激活后，ＨＯ－ ８９１０ 细胞与其的粘附较激活前增加３ 倍以上。用抗Ｅ－ ｓｅｌｅｃｔｉｎ 单抗处理血管内皮细胞或用其配体ｓＬｅｘ 的单抗处理肿瘤细胞，可分别将两细胞的粘附率阻断６２－５ ％ 和８１ ％ 。结论：Ｅ－ ｓｅｌｅｃｔｉｎ 和它的配体ｓＬｅｘ 是介导激活的血管内皮细胞与ＨＯ－ ８９１０
AIM and METHODS: To study the mechanism of hematogenous metastases of human HO-8910 ovarian cancer cells, the adhesion of HO-8910 cells to cultured human vascular endothelial cells (HUVECs) activated by LPS and the effects of monoclonal antibodies (mAb) against E-selectin and its ligand sLe x on the adhesive interaction have been studied by a method for detecting tumor cells uptaking Rose Bengal. RESULTS:Treatment of HUVECs monolayers with LPS (1 μg/mL) for 6 hours caused more than 3-fold increase of the adhesion of HO-8910 cells to HUVECs( P<0.01 ) and HO-8910 cells with high metastatic capacity showed higher adhesive ability to HUVECs than that of human colorectal cancer HR-8348 cells with low metastatic capacity. 62.5% and 81% adhesion between HO-8910 and activated HUVECs could be blocked by anti-E-selectin and anti-sLe x mAbs, respectively. CONCLUSION:E-selectin-sLe x play an important role in mediating the adhesive reaction of HO-8910 cells to activated vascular endothelium.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第11期975-977,共3页
Chinese Journal of Pathophysiology
