摘要
目的 以逆转录聚合酶链反应 (RT PCR)检测外周血肺癌细胞 ,以监测其血路转移情况。方法 应用RT PCR方法检测 2 5例肺癌手术患者和 3 5例肺癌化疗前患者外周血中细胞角蛋白2 0 (CK2 0 ) ,并以肺癌组织和非肿瘤患者外周血为对照。结果 非肿瘤患者外周血中基本上无CK2 0检出 ,肺癌手术时肺动脉血中CK2 0检出明显升高 ,阳性率达 5 4 % ( 13 / 2 5 )。晚期肺癌患者CK2 0表达亦较高 ,阳性率达 4 0 % ( 10 / 2 5 )。结论 CK2 0可能作为肿瘤标志物 ,检测外周血中的肺癌细胞及用于预测肿瘤转移复发。
Objective To detect peripheral cancer cells by reverse transcriptase polymerase chain reaction (RT PCR)for monitoring metastasis in blood. Methods Twenty five patients with lung cancer receiving operation and 35 patients having pre chemotherapy were determined by RT PCR cytokeretin 20 (CK20) of cancer cells in the peripheral blood in contrast to that of the tissue of lung cancer and the peripheral blood of patients with non tumor. Results In the operative group, 13 patients (54%) showed positive CK20 in artery blood and 10 (40%) positive CK20. In the peripheral blood of patients with nontumor, CK20 was negative. CK8 and CK19 were rarely positive. Conclusion CK20 may be a tumor marker for detecting cancer cells in peripheral blood as well as tumor metastasis and relapse.
出处
《中华检验医学杂志》
CAS
CSCD
2000年第1期17-18,共2页
Chinese Journal of Laboratory Medicine
