摘要
目的探讨临床分离多药耐药大肠埃希菌(ECO)的Ⅰ类整合子流行分布及与多药耐药之间可能存在的关系。方法采用纸片扩散法(K-B法)检测71株大肠埃希菌对3类4种常用抗菌药物的药敏率;PCR方法检测Ⅰ类整合子3个不同部位的基因。结果 71株大肠埃希菌耐庆大霉素47株为66.20%、耐左氧氟沙星45株为63.38%、耐头孢噻肟42株为59.15%、耐头孢他啶13株为18.31%,多药耐药率为32.39%;Ⅰ类整合子的总检出率为88.73%,整合子阳性与阴性株对庆大霉素、左氧氟沙星、头孢噻肟的药敏分布差异有统计学意义(P<0.05),多药耐药菌株中Ⅰ类整合子基因的阳性率为100.0%,耐多药菌株与全敏感菌株差异有统计学意义(P<0.05)。结论大肠埃希菌的Ⅰ类整合子的检出率高,且与其多药耐药性密切相关。
OBJECTIVE To investigate the distribution of type I integron in local multidrug resistant Escherichia coli（E, coli）, to analyze the possible relationship between the type I integron and multidrug resistance. METHODS The susceptibilities of 71 E. coli strains to 4 antibiotics were detected by disk diffusion test. IntI1 gene, variable region,qacEDeltal-sull gene, which were in 3 different parts in type I integron, were detected bY PCR. RESULTS Totally 47 strains（66.20%） were resistant to gentamicin, 45 strains （63.38%） to levofloxacin, 42 strains（59. 15%）to cefotaxime, and 13 strains（18. 31%）to ceftazidime. There were 23 （32. 39%）multidrug resistant strains in 71 E. coli strains. The positive rate of type I integron 71 E. coli strains was 88.73%. The constituent ratio of susceptibility to gentamicin, levofloxacin and cefotaxime in integron-positive strains was statistically different from that in integron-negative strains. The type I integron-positive rates were 100. 00% in multidrug resistant, which were statistically different with that in sensitive strains （P〈0.05）. CONCLUSION The high detection rate of type I integron in E. coli is high, which is cosely associated with the multidrug resistance.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第14期2882-2885,共4页
Chinese Journal of Nosocomiology
基金
四川省重点学科重点建设项目资助(SZD0241)
关键词
大肠埃希菌
多药耐药
I类整合子
Escherichia coli
Multidrug resistance, Type I integron
