摘要
利用矮生型进行密植栽培是桃树生产发展的一个重要方向。利用SRAP标记技术以寿星桃A20(Prunuspersica var.densa Makino)与普通桃(P.persica L.Batsch)品系99-12-34的F1代杂交分离群体共123个单株(矮生型63株,普通型60株)为试材,对控制桃高/矮性状的基因进行分子定位,从而为明确桃的矮化机理提供依据。用分离群体分组分析法(Bulked Segregant Analysis,BSA),通过对336对SRAP引物的筛选,获得了1个与高/矮性状连锁距离为3.2 cM的SRAP标记。利用该标记对本试验群体以外的34个桃品种的DNA进行扩增,符合率达到88.2%,初步认为可以用于桃的分子辅助育种。
Close planting by brachytic dwarf peach is one of the main direction for peach industry.About 123（1-year-old） hybrid individuals derived from the cross of Shouxing Tao peach（Prunus persica var.densa Makino） A20 and peach [P.persica（L.） Batsch] 99-12-34 which was used to identify SRAP markers linked to the character of tall normal/brachytic dwarf for understanding of the dwarfing mechanism.The individuals were divided into two types.About 63 individuals have the character of brachytic dwarf and 60 individuals have the character of tall normal.Altogether,336 pairs of SRAP primers were screened by bulked segregant analysis（BSA）.Finally,a SRAP marker,Me11Em25,which linked to the character of tall normal/brachytic dwarf at a distance of 3.2 cM,was selected.The marker wea used to test in 34 varieties and has the coincidence rate of 88.2%.The results showed the marker could be used for molecular assisted selection（MAS） in peach.
出处
《果树学报》
CAS
CSCD
北大核心
2011年第4期586-590,共5页
Journal of Fruit Science
基金
国家自然科学基金(30871681)
863项目(2006AA100108-4-12)
江苏省农业科学院学科带头人优秀后备人才(6510822)
