摘要
以多壁碳纳米管为吸附材料,建立基质固相分散萃取高效液相色谱法测定牛奶中6种四环素。将多壁碳纳米管、EDTA、草酸和样品研磨混匀装柱,经含甲酸的甲醇洗脱后HPLC多波长紫外检测。6种四环素的检出限0.01～0.03μg/mL,回收率78.7%～105.2%,相对标准偏差小于12%。多壁碳纳米管基质固相分散萃取牛奶中四环素的回收率优于C18,可推广到食品等复杂样品中其他农兽药多残留分析。
A matrix solid-phase dispersion extraction （MSPDE） method was developed to extract 6 tetracycline antibiotics （TCs） from milk samples using multiwalled carbon nanotubes （MWCNTs） as adsorbent prior to high performance liquid chromatography （HPLC） determination. Milk samples, MWCNTs, oxalic acid, and EDTA were evenly ground and then packed into a glass syringe as dispersion column. The analytes were eluted with methanol containing formic acid, and detected by HPLC with variable wavelength detector at 365 nm. The limits of detection （LODs） for 6 TCs ranged from 0.01 to 0.03 μ g/mL. Their recoveries in spiked milk samples were in the range of 78.7% - 105.2% with relative standard deviations of less than 12%. MSPD exhibited higher recovery in extracting 6 TCs as compared to C18 column extraction, suggesting that the developed MSPD procedure can be applied for the multiresidues analysis of pesticides and veterinary drugs in foods.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第14期206-209,共4页
Food Science
基金
国家质量技术监督检验检疫总局项目(2009IK131)
江苏省自然科学基金项目(BK2008121)
徐州市科技计划项目(XM08C026)
关键词
四环素
基质固相分散
碳纳米管
高效液相色谱法
tetracycline antibiotics
matrix solid-phase dispersion extraction
carbon nanotubes
high performance liquid chromatography
