摘要
双螺杆挤出改性处理脱脂玉米蛋白粉。以脱脂玉米蛋白粉含水量、挤出温度、螺杆转速为响应因素,可溶性蛋白含量为响应值,采用响应面分析方法,确定脱脂玉米蛋白粉挤出改性的最佳工艺条件。结果表明:物料含水量68%、挤出温度163℃、螺杆转速30Hz(225r/min)时,挤出脱脂玉米蛋白粉中可溶性蛋白含量最高,为3.43%。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定挤出改性后玉米可溶性蛋白的分子质量分布,结果显示其分子质量介于3500～14300D之间,且主要是分子质量为3500～6500D的多肽。改性后的玉米蛋白粉其理化性质都有了较明显的改善,说明双螺杆挤出改性玉米蛋白粉效果明显,切实可行。
The objective of the present study was to optimize the process conditions for the modification of defatted corn gluten meal by two-screw extrusion by response surface methodology. The soluble protein content of extruded corn gluten meal was investigated with respect to water content before extrusion, extrusion temperature and screw rotation speed. The results indicated that the optimal twin-screw extrusion conditions were water content of 68%, extrusion temperature of 163 ℃ and rotation speed of 30 Hz （225 r/min）. Under the optimal conditions, the soluble protein content of extruded corn gluten meal reached up to 3.43%. SDS-PAGE analysis demonstrated that the molecular weight distribution of soluble proteins in extruded corn gluten meal was in the range of 3500- 14300 D, mainly consisting of polypeptides with a molecular weight between 3500 D and 6500 D. Modified corn gluten meal showed an obvious improvement in its physicochemical properties. Therefore, twinscrew extrusion is feasible to produce modified corn gluten meal.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第14期72-78,共7页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAD27B07)
关键词
脱脂玉米蛋白粉
双螺杆挤出
改性
defatted corn gluten meal： twin-screw extrusion
modification
