摘要
目的:研究疏肝健脾方对腹泻型肠易激综合征(D-IBS)大鼠血及结肠黏膜中5-羟色胺(5-HT)、降钙素基因相关肽(CGRP)的影响,探讨其疗效机制。方法:60只Wistar大鼠随机分为正常组,模型组,疏肝健脾方高、中、低剂量组和匹维溴胺组;用寒冷-束缚刺激法复制D-IBS大鼠模型;采用紫外分光光度法测各组大鼠血清5-HT含量,放免法检测血浆CGRP水平,免疫组织化学SABC法检测结肠黏膜组织5-HT,CGRP的表达情况。结果:模型组大鼠血中5-HT含量及结肠黏膜中5-HT阳性纤维的平均吸光度(A)升高,CGRP降低,与正常组比较,差异有统计学意义(P<0.05);各用药组血中5-HT及其在肠黏膜的表达降低,CGRP升高,与模型组比较,差异有统计学意义(P<0.05),且以疏肝健脾高、中剂量组差异明显(P<0.01)。结论:疏肝健脾方通过调节D-IBS大鼠5-HT,CGRP的变化,是其发挥疗效的作用机制之一。
Objective：To observe the effect of Shugan Jianpi Recipe on 5-hydroxytryptamine（5-HT）,calcitonin gene related peptide（CGRP） in rats with diarrhea predominant irritable bowel syndrome（D-IBS）.Method： Sixty Wistar rats were randomized into normal group,model group,low,middle and high-dose groups of Shugan Jianpi Recipe,and dicetel group.Cold-binding applications were given to establish similar model of D-IBS.Violet spectrophotometer was used for determining the levels of 5-HT in serum.RIA was used for measuring the levels of CGRP in serum.The expressions of 5-HT and CGRP in intestinal and colon mucosa and colon tissue were detected by immunohistochemical SABC method.Result： In the model group,the 5-HT level increased,CGRP decreased,compared with those in the normal group（P0.05）.The Shugan Jianpi Recipe＇s middle,high-dose increased 5-HT and decreased CGRP significantly,compared with that in the model group（P0.01）.Conclusion： The therapeutic mechanism of Shugan Jianpi Recipe for D-IBS is probably related with adjustment of 5-HT and CGRP in serum and colon tissue.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第14期179-181,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
疏肝健脾方
腹泻型肠易激综合征
5-羟色胺
降钙素基因相关肽
结肠黏膜
大鼠
Shugan Jianpi Recipe
diarrhea predominant irritable bowel syndrome
5-hydroxytryptamine
calcitonin gene related peptide
colonic mucosa
rats
