摘要
目的:研究大孔树脂纯化二仙汤有效部位群的最佳条件。方法:以复方二仙汤中总黄酮、总生物碱、总皂苷为指标,以动态法考察D101,DM130,HPD100,HPD400 4种树脂对各指标的吸附和解析性能,优选出适宜的树脂,并进一步优化其吸附与解析条件。结果:D101型树脂较适宜纯化复方二仙汤有效部位群,最佳条件为二仙汤调药液比1∶1.5,加NaCl调至1.5mol.L-1,以2.5 BV上样,6 BV水洗至近无色,再用5 BV的50%乙醇洗脱,得有效部位群。结论:D101型树脂纯化复方二仙汤有效部位群工艺简单可行。
Objective：To optimize the purification condition of active ingredients in Er-xian decotion with macroporous resin.Method： With the total flavonoids,total alkaloids and total saponins in Er-xian decotion as parameters,the D101,DM130,HPD100,HPD400 resins were inspected by dynamic method for the adsorption and desorption performance to screen the best resin and optimize the condition of absorption and desorption for the resin.Result： The D101 resin was suitable to purify the active ingredients in Er-xian decoction.The optimized condition is that： The ratio of medicine to solution is 1∶1.5,NaCl concentration is 1.5 mol·L-1,loading 2.5 BV medicine to D101 resin column,eluting to colourless with 6 BV water,and finally,the active ingredients were eluted by 50% ethanol（5 BV）.Conclusion： The active ingredients in Er-xian decotion can be purified simply and practically by D101 resin.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第14期23-26,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省教育厅青年基金(GJJ10279)
关键词
二仙汤有效部位群
大孔树脂
吸附分离
active ingredients in Er-xian decotion
macroporous resin
adsorption and separation
