摘要
多聚磷酸盐(polyP)是由几个到几百个无机磷酸盐单体通过高能磷酸键聚合而成的线性多聚体,广泛分布于自然界和生物体。本文总结了polyP在生物体中的重要功能,包括基因表达和调控、DNA的摄取、微生物的运动性、对胁迫和饥饿的应答、病原菌的毒性以及对细胞凋亡、血液凝固、细胞钙化、线粒体功能的调节,需要polyP的酶有内切酶、葡萄糖激酶、NAD激酶和AMP磷酸转移酶等。本文对调控polyP的多聚磷酸盐激酶(polyphosphate kinase,ppk)和外切聚磷酸酶(ex-opolyphosphatase,PPX)的生化性质和结构也进行了总结。同时,结合我们的研究工作,重点分析了结核分枝杆菌中PPX的同源蛋白和可能的生物化学活性。
Inorganic polyphosphate（Poly P） is a polymer consisting of ten to hundreds of phosphate residues linked by ＂high-energy＂ phosphoanhydride bonds,which is abundantly found in all organisms and nature.Here the basic facts of PolyP are summarized： genes regulated by polyP,role in DNA uptake,motility of microorganism,function in stress response,the virulence of pathogens,as well as the proliferation of mammary cancer cells,blood coagulation,cell calcification and the modulation of mitochondrial activity.Enzymes with activities requiring polyP,such as endopolyphosphatase,glucokinase,NAD kinase,AMP phosphotransferase are outlined too.The structure and activity of enzymes regulating polyP level such as polyphosphate kinase and exopolyphosphatase are noted.A thorough analysis of the mycobacterium tuberculosis PPX protein homologs and their biochemical activity is presented.
出处
《生理科学进展》
CAS
CSCD
北大核心
2011年第3期181-187,共7页
Progress in Physiological Sciences
基金
国家重要传染病十一五科技重大专项(2008ZX10003-006
2008ZX10003-001)
中央高校基本科研业务费专项资金(XDJK2009A003)
西南大学研究生科技创新基金项目(kb2009010ky2009009)
重庆市科委自然科学基金计划(CSTC
2010BB5002)资助项目
