摘要
目的探讨标本中溶血、脂血、血液保存液对抗-HIV ELISA法检测结果的影响。方法采用模拟对照实验,用溶血、脂血、血液保存液和小牛血清分别对抗-HIV阳性质控血清进行系列梯度稀释,用ELISA法检测,观察溶血、脂血、血液保存液对检测结果的影响。结果含有血液保存液的抗-HIV阳性质控血清的检测结果均明显低于小牛血清对照组(P<0.01),且在60%稀释度内,随血液保存液浓度逐步增加,降幅有加大趋势;而溶血、脂血对抗-HIV阳性质控血清的检测结果影响差异无统计学意义(P>0.05)。结论血液保存液对ELISA法检测抗-HIV结果影响明显;而溶血、脂血对抗-HIV ELISA法检测结果无影响。
Objective To investigate the influence of hemolysis,fat and blood preservation solution on the results of the ELISA detection of anti-HIV. Methods Quality control serum, positive with anti-HIV,were diluted serially with hemolytic plasma, plasma with fat,blood preservation solution and calf serum, respectively, then the serial dilutions were detected for anti HIV by ELISA. Then the influence of the factors, mentioned above, was observed. Results The detection result of quality control serum, diluted with blood preservation solution, was significantly lower than that of quality control serum,diluted with calf serum （P〈0.01）, and within the dilute strength of 60% ,decreasing degree of the detection result gradually increased with the increase of the concentration of blood preservation solution. There was no statistical difference of the influence between hemolysis and fat on the detection result of （P〈0.05）. Conclusion The blood preservation solution significantly influence the ELISA detection of anti-HIV,but he- molysis and fat have no influence.
出处
《国际检验医学杂志》
CAS
2011年第10期1050-1051,共2页
International Journal of Laboratory Medicine
关键词
HIV抗体
酶联免疫吸附测定
血液保存液
影响
HIV antibodies
enzyme-linked immunosorbent assay
blood preservation solution
influence
