摘要
目的探讨PTEN mRNA和SurvivinmRNA在膀胱移行细胞癌中的表达及其与病理分级、临床分期和复发的关系。方法采用SYBRGreenII实时荧光定量逆转录聚合酶链反应试验方法检测43例膀胱癌组织及9例膀胱正常组织中PTENmRNA和SurvivinmRNA的表达情况。结果膀胱正常组织中PTENmRNA的表达高于膀胱癌组织，SurvivinmRNA表达高于膀胱癌组织，两者比较差异有统计学意义（P〈0.05）；PTENmRNA和SurvivinmRNA在膀胱癌低级别和高级别、表浅型和浸润型、初发和复发中的表达比较差异均有统计学意义（P〈0．05）。PTENmRNA和SurvivinmRNA在膀胱癌中的表达成负相关（r=0．972，P〈0．05）。结论IYFEN和Survivin参与了膀胱癌的发生发展过程，对膀胱癌的诊断、判断病理分级。临床分期和预后具有重要临床意义，膀胱癌中PTEN和Survivin两者mRNA的表达呈负相关，二者表达的失调可能是BTCC的发生发展的机制之一。
Objectives To study expression of PTEN mRNA 和 Survivin mRNA in BTCC and its relationship between BTCC and normal tissue ,clinical stage ,pathology grade and recurrence. Methods The SYBR Green II real time quantitative RT - PCR was used to evaluated the expression of PTEN mRNA 和 Surcivin mRNA in 43 cases of transitional cell carcinoma of urinary bladder and 9 normal cases. Results The expression of PTEN mRNA in normol tissue is higher than that in BTCC, and The expression of Survivin mRNA in normal tissue is lower than that in BTCC There were statistical significance between normol tissue and BTCCo There were also statistical significance between the grade （ p 〈 0.05 ） , the clinical stage （ p 〈 0.05 ） , and primary and recurrence （ p 〈 0. 05 ）. There was a negative correlation between the expression of PTEN mRNA 和 Survivin mRNA in BTCC （r = -0. 972, p 〈 0.05 ）. Conclusions FFEN mRNA 和 Survivin mRNA may be implicated in the patnogenesis of BTCC, and they play role in diagnosing BTCC ,judging pathological grade and clinical stage and have important clinical significance. The imbalance expression of them may be a reason of the original and developong mechanism of BTCC.
出处
《国际泌尿系统杂志》
2011年第4期452-455,共4页
International Journal of Urology and Nephrology
关键词
膀胱肿瘤
Bladder Neoplasms
