摘要
本实验旨在研究姜黄醇提物及其制剂中总姜黄素含量的测定及方法学考察。利用姜黄素及其同系物在λ426nm处有一吸收峰而取样直接比色测定。该方法操作简便，相关性好，R≥0.9995，线性范围在1.25～6.25ug／ml，经过对姜黄醇提物及其胶囊的几次重复测定，重视性RSD＜2．5％，样品回收率99．3％±1．00％．并测得三批姜黄醇提物纯度在61．13％～67．45％，胶囊中总姜黄素含量23．43％～23．82％。
This experiment aims to study the measurement of the total contents of curcumin ln turmerol extract and its pharmaceutics and methodological observation. Take sample at A 426～429nm where curcumin and its homologen have an absorptive summit and directly make a colorimetric determination. This method is easy to control, has good correlation, its R>0. 9995 - the lineal range is 1. 25 ～ 6. 25ug/ml. By several repeating measurement of turmerol extract and its capsule, the reproducibility (RSD) < 2. 5%, the rate of sample recovery is 99. 3 % ±1.00 %,and the purity of three turmerol extracts is 61. 13 %～67- 45 %,the total content of curcumin is 23. 43 %～23. 82 %.
出处
《浙江中医学院学报》
1999年第6期24-25,共2页
Journal of Zhejiang College of Traditional Chinese Medicine
基金
国家中医药管理局科研基金!92B049
关键词
姜黄素
含量
中药
分光光度法
curcumin
colorimetric method
content measurement
methodology
