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高糖培养对雪旺细胞增殖及血管内皮细胞生长因子分泌的影响 被引量:4

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摘要 目的:通过检测高糖培养下雪旺细胞增殖及血管内皮细胞生长因子(VEGF)分泌量的改变,探讨高糖对雪旺细胞的影响。方法:在葡萄糖浓度分别为10、30、60mm/L的培养基中培养雪旺细胞,分别于24、48、72h,用CCK-8检测雪旺细胞增殖,ELISA方法检测细胞培养液VEGF浓度。结果:高糖培养后,雪旺细胞比正常葡萄糖培养,增殖速度减慢,VEGF分泌减少。结论:高糖抑制雪旺细胞增殖,VEGF分泌减少,对周围神经修复过程有损害。
出处 《实用医学杂志》 CAS 北大核心 2011年第13期2325-2326,共2页 The Journal of Practical Medicine
基金 广东省科技计划项目(编号:2007B031506001)
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同被引文献67

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