摘要
目的：分析比较干细胞因子（ＳＣＦ）、ＴＧＦ－α及某些膜蛋白、核蛋白基因在不同病理状态下血管平滑肌细胞中的表达水平，探讨这些基因产物在动脉粥样硬化及高血压发病过程中所起的作用。方法：从维生素Ｄ３ 诱导动脉粥样硬化和一侧肾动脉结扎的高血压大鼠取出主动脉，在体外培养收集血管平滑肌细胞，抽提细胞总ＲＮＡ，以同位素标记的核酸探针作Ｎｏｒｔｈｅｒｎ ｂｌｏｔ分析。结果：动脉粥样硬化及高血压血管平滑肌细胞中干细胞因子ｍ ＲＮＡ 表达比正常时高出许多倍，ＴＧＦα在高血压血管平滑肌细胞中有较高表达，而ｅｒｂＢ２ 表达无明显变化。结论：生长因子在血管的损伤修复中起重要作用，实验提示ＳＣＦ参与了动脉粥样硬化和高血压时动脉平滑肌细胞的增殖调控过程，可能通过干细胞因子这一系统参与血管损伤修复。
Aim:To explore factors influencing on atherosclerotic and hypertension progression,the mRNA expression levels of stem cell factor(SCF),transform growth factor α(TGFα),some membrane proteins and nuclear proteins of vascular smooth muscle cells(VSMCs)in different pathologic conditions were analysed. Methods:Atherosclerosis of rat was induced by vitamin D 3 and hypertension model was prepared by patially obstraction of left renal artery. Total RNA were extracted from cultured vascular smooth muscle cells and analysed with Northern blot. Results:The high level expression of two type of SCF mRNA and incremental TGF α mRNA were found in atherosclerotic and hypertensive vascular smooth muscle cells. Conclusion:Growth factors play an important role in the repair of blood vessel. It is suggested that SCF involved the proliferation control of VSMCs in the atherosclerosis and hypertension by SCF pathway.
出处
《高血压杂志》
CSCD
1999年第4期363-365,共3页
Chinese Journal of Hypertension
关键词
动脉粥样硬化
SCF
血管平滑肌细胞
stem cell factor
\ vascular smooth muscle cell
\ gene expression
