摘要
通过比较了6种大孔吸附树脂D4006、NKA-9、X-5、HPD450、HPD600和AB-8对花椒总黄酮的吸附及脱附性能,筛选出最佳树脂进行动态实验研究,得到优化的纯化条件。结果表明:AB-8型大孔树脂纯化效果最好,其最佳工艺为:上样浓度3.0～4.0mg/mL,上柱pH4.5左右,上柱速度2.5mL/min,洗脱剂最适宜体积分数与洗脱剂用量分别为70%和3BV(柱体积bed volume)。经AB-8型大孔吸附树脂纯化后,花椒黄酮的纯度由51.46%提高到90.25%,总黄酮回收率81.65%。对经AB-8大孔树脂纯化后的花椒黄酮进行高效液相色谱(HPLC)分析发现,黄酮纯度显著提高,其中芦丁含量较高。
Adsorption and desorption characteristics of macroporous adsorption resins D4006, NKA-9, X-5, HPD450,HPD600 and AB-8 toward flavones of Zanthoxylum bungeanum were investigated.The best macroporous adsorption resin was chosen to carry out dynamic adsorption experiments, while the purification process was acquired.The results showed that the AB-8 macroporous resin gave the best effect of separation with concent ratio 3.0-4.0mg/mL,the feed solution pH 4.5 and the sampling rate 2.5mL/min, respectively, and the concent ratio of elution was 70% alcohol, with eluant volume 3BV （ bed volume）. By this technology the flavonoid content was increased from 51.46% of the crude extract to 90.25% of the purified product and the yield was 81.65%. High performance liquid chromatography （HPLC） analysis result showed that the purity of obtained flavonoids was significantly increased,and the rutin content was higher than other flavonoids.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第7期260-262,266,共4页
Science and Technology of Food Industry
基金
国家"十一五"科技支撑计划重点课题(2006BAD06B02)
关键词
花椒
黄酮
大孔吸附树脂
纯化
Zanthoxylum bungeanum
flavonoid
macroporous resin
purification
