摘要
目的探讨苦参碱(Mat)对体外培养的兔晶状体上皮细胞(RLECs)内Ⅰ型和Ⅲ型胶原蛋白合成的影响。方法 RLECs培养后分为3组:空白对照组以DMEM为培养基;重组人表皮生长因子(rhEGF)组以DMEM+50μg/L rhEGF为培养基;Mat组以含50μg/L rhEGF和1.0 g/L Mat的DMEM为培养基。利用50μg/LrhEGF诱导RLECs增殖,加入1.0 g/L Mat培养24 h后,采用酶联免疫吸附测定法(ELISA)检测RLECs内Ⅰ型和Ⅲ型胶原蛋白表达。结果 50μg/L rhEGF作用后,RLECs内Ⅰ型和Ⅲ型胶原蛋白浓度分别为57.65±4.54μg/L和1.59±0.19μg/L,较空白对照组明显升高(P<0.01)。Mat作用后,RLECs内Ⅰ型和Ⅲ型胶原蛋白浓度分别为21.74±3.96μg/L和0.56±0.21μg/L,较rhbFGF组和空白对照组明显下降(P<0.01)。结论 Mat能抑制体外培养的RLECs内Ⅰ型和Ⅲ型胶原蛋白的合成。
Objective To study the effects of Matrine（Mat）on collagen synthesis of rabbit lens epithelial cells（RLECs）in vitro.Methods Cultured RLECs were divided into three groups：control group was added DMEM,rhEGF group was added DMEM and 50 μg/L rhEGF,Mat group was added DMEM,50 μg/L rhEGF and 1.0 g/L Mat.Using 50 μg/L rhEGF to induce proliferation of RLECs.Proliferative RLECs were incubated with 1.0 g/L Mat for 24 hours.The contents of collagen Ⅰ and Ⅲ of RLECs were assayed by enzyme-linked immunosorbent assay（ELISA）.Results The contents of collagen Ⅰ and Ⅲ of RLECs in rhEGF group were 57.65±4.54 μg/L and 1.59±0.19 μg/L respectively,which increased obviously compared with control group（P〈0.01）.The contents of collagen Ⅰ and Ⅲ of RLECs in1.0 g/L Mat group were 21.74±3.96 μg/L and 0.56±0.21 μg/L respectively,which decreased obviously compared with control group and rhEGF group（P〈0.01）.Conclusion Mat could effectively inhibit the sythesis of collagen Ⅰ and Ⅲ of RLECs in vitro.
出处
《中南医学科学杂志》
CAS
2011年第2期165-167,共3页
Medical Science Journal of Central South China
关键词
苦参碱
晶状体上皮细胞
胶原蛋白
后囊膜混浊
兔
Matrine
lens epithelial cell
collagen
posterior capsular opacification
rabbit
