摘要
研究发现在碱性条件下,纳米银对鲁米诺-铁氰化钾液相化学发光体系发光信号具有明显的增敏作用,而4-乙酰氨基酚对该体系具有强烈的抑制作用。结合流动注射技术,建立了流动注射化学发光分析法测定对乙酰氨基酚的新方法。该方法测定对乙酰氨基酚的线性范围为9.0×10-12～1.0×10-10g/mL(0.9947)和1.0×10-10～7.0×10-9g/mL(0.9904),检出限(3σ)为7.9×10-12g/mL。对5.0×10-11g/mL和5.0×10-10g/mL对乙酰氨基酚平行测定11次,其相对标准偏差为1.3%和1.3%。该方法用于片剂中对乙酰氨基酚的测定,回收率为97.0%～101.5%。
The experiments indicated that silver nanoparticles（AgNPs） could greatly enhance the chemiluminescence intensity of the luminol-K3Fe（CN）6 system in sodium hydroxide medium,but paracetamol could inhibit the chemiluminescence signals significantly.On the basis of the inhibition,a novel flow-injection chemiluminescence method for the determination of paracetamol was proposed.The experimental conditions that influence the chemiluminescence intensity were carefully studied and optimized.Under the optimum conditions,the calibration graph for paracetamol was obtained.The linear range for the determination of paracetamol was from 9×10-12 to 1.0×10-10g/mL（0.9947） and 1.0×10-10 to 7.0×10-9 g/mL（0.9904） with a detection limit of 7.9×10-12 g/mL.The relative standard deviations for 11 repeated measurements of 5.0×10-11 g/mL and 5.0×10-10g/mL paracetamol were 1.3% and 1.3%,respectively.Possible interferences from major excipients in pharmaceuticals and other related compounds were also studied.Contents of paracetamol in two locally available pharmaceuticals were analyzed.The method has the merits of rapidness,simplicity,high sensitivity,wide linear range and has been used for the analysis of paracetamol in commercial tablets with recoveries in the range from 97.0% to 101.5%.
出处
《分析试验室》
CAS
CSCD
北大核心
2011年第7期26-30,共5页
Chinese Journal of Analysis Laboratory
基金
陕西省教育厅专项科研基金(09JK821)
延安大学研究生教育创新计划项目(YJS2010-22)资助
关键词
鲁米诺
纳米银
液相化学发光体系
流动注射
对乙酰氨基酚
Luminol
Silver nanoparticles
Liquid chemiluminescence system
Flow-injection
Paracetamol
