摘要
建立反相高效液相色谱法同时测定不同根龄虎杖根中虎杖苷和白藜芦醇含量。采用KromasilC18（4.6mm×250mm,5μm）色谱柱,流动相为甲醇-1.0%磷酸水溶液（50：50,v/v）,流速0.8mL/min,检测波长319nm,柱温为30℃。虎杖苷和白藜芦醇的进样量分别在0.716~7.160μg（r=0.9996）和0.740~7.400μg（r=0.9998）范围内与峰面积呈良好的线性关系;平均回收率（n=6）分别为99.7%和98.5%。本方法快速简便,重复性好,专属性强,可作为虎杖根的质量控制方法。
A reserved-phase HPLC method for the content determination of erbascoside and resveratrol in root of Polygonum cuspidatum was established. The analytical was carried out with Kromasil C18 （4.6 mm×250 mm, 5 μm） column and the mobile phase consisted of acetonitrile-1.0 % phosphoric acid aqueous solution （50：50,v/v） at a flow rate of 0.8 mL/min. The detection wavelength was 319 nm and the column temperature was set at 30 ℃. The calibration curves were linear within the range of 0.716~7.160 μg （r=0.9996） for erbascoside and 0.740~7.400 μg （r=0.9998） for resveratrol; The average recoveries were 99.7 % for erbascoside and 98.5 % for resveratrol. The method is simple, accurate, reproducible and precise, and applicable for the determination of erbascoside and resveratrol in different age root of Polygonum cuspidatum.
出处
《生命科学仪器》
2011年第3期37-39,共3页
Life Science Instruments
