摘要
目的探讨雌激素对血管性痴呆(VD)大鼠认知功能及海马CA1区胰岛素样生长因子-1(IGF-1)、胆碱乙酰转移酶(ChAT)表达的影响。方法 30只雄性SD大鼠随机分为假手术组、VD组和雌激素组,每组10只。采用双侧颈总动脉结扎(2VO)法制备VD大鼠模型;雌激素组大鼠腹腔注射17-β雌二醇(花生油溶解)1 mg/kg,假手术组和VD组大鼠腹腔注射等量的花生油,均为隔日1次,共30次。60 d后,用Morris水迷宫检测各组大鼠逃避潜伏期和跨越平台次数;HE染色观察大脑海马CA1区神经细胞形态学变化,免疫组化染色检测海马CA1区IGF-1、ChAT阳性细胞数。结果假手术组海马CA1区未见明显的病理变化;VD组神经细胞数量和层次明显减少;雌激素组偶见小的软化灶,细胞数和形态接近假手术组。与假手术组相比,VD组及雌激素组逃避潜伏期明显延长,跨越平台次数明显减少,海马CA1区IGF-1表达明显增加,ChAT表达明显减少(均P<0.05);与VD组比较,雌激素组逃避潜伏期明显缩短,跨越平台次数明显增加,海马CA1区IGF-1及ChAT表达明显增加(均P<0.05)。结论雌激素可以通过上调内源性IGF-1而减轻海马神经元损伤,增加ChAT的表达,改善VD大鼠认知功能。
Objective To explore the effects of estrogen on cognitive function and the expression of insulin like growth factor-1 （IGF-1）, choline acetyltransferase （CHAT） in hippocampus CA1 region in rats with vascular dementia （VD）. Methods Thirty male SD rats were randomly divided into sham-operated group, VD group and estrogen group, 10 rats in each group. VD models were made with permanent bilateral common carotid arteries occlusion （2VO）. The 17-[3 estradiol （1 mg/kg, dissolution in peanut oil） was injected in intraperitoneal in estrogen group; while, same-dose peanut oil was injected in intraperitoneal in the sham-operated group and VD group. The injection in the 3 groups was once every other day, total 30 times. After 60 d, the escape latency and number of crossing original platform were examined by Morris water maze test. Neurons morphologie change in hippoeampus CAI region was observed by HE stain; the quantity of IGF-1 and ChAT positive cells were detected by immunohistoehemistry. Results In hippoeampus CA1 region, no pathologic change was found out in sham-operated group ; the quantity and layer of neurons were significantly decreased in VD group ; the small local malaeosis could be found in estrogen group, but the quantity and form of neuroeyte were like as sham-operated group. Compared to sham- operated group, in VD group and estrogen group, the escape lateneies were significantly delay, the number of crossing original platform were significantly decreased; the expression of IGF-1 were increased and ChAT was decreased in hippocampus CA1 region （all P 〈 0. 05 ）. Compared with VD group, the escape latency was significantly shorter, the number of crossing original platform was significantly increased; the expression of IGF-1 and ChAT in hippoeampus CA 1 region were significantly increased obviously in estrogen group （ all P 〈 0. 05 ）. Conclusion By up regular the expression of endogenous IGF-1, estrogen can reduce the damage of hippoeampal neurons, and increase the expression of CHAT, and improver cognitive function in VD rats.
出处
《临床神经病学杂志》
CAS
北大核心
2011年第3期194-197,共4页
Journal of Clinical Neurology
