摘要
目的探讨缺血预处理对沙土鼠脑缺血再灌注后神经细胞凋亡的影响。方法采用阻断双侧颈总动脉制作沙土鼠全脑缺血模型,96只沙土鼠,腹腔注射戊巴比妥钠麻醉。沙土鼠随机分为四组:SH组:假手术对照组,未行脑缺血预处理;IC组:预处理对照组,予2 min脑缺血后未再次缺血;IP组:预处理缺血组,予2 min脑缺血,再灌注24 h后,再次脑缺血10 min;IR组:脑缺血再灌注组,予脑缺血10 min后行再灌注。每组据再灌时间点的不同又分为Ⅰ(1d)、Ⅱ(3d)、Ⅲ(5d)、Ⅳ(7d)4个亚组,每个亚组6只动物。采用TUNEL法检测沙土鼠脑海马CA1区神经细胞凋亡数目。结果IP可显著减少沙土鼠脑海马CA1区神经细胞凋亡数量(与IR组相比P<0.01)。结论脑缺血预处理可显著提高机体对后续缺血的耐受性,其机制可能是通过抑制缺血诱导的神经细胞凋亡而发挥脑保护作用。
Objective： To investigate the influence of the cerebral ischemic preconditioning on the neural apoptosis following ischemia and repeffusion in gerbils, Methods- Cerebral ischemia was induced by occlusion of bilateral common carotid arteries. Gerbils were randomly divided into four groups- Sham- operation control group （SH） ： without cerebral ischemia; Cerebral ischemic preconditioning control group （IC） ; 2 rain cerebral ischemia only; Cerebral ischemic preconditioning test group （IP） - 2 min cerebral ischemia and 24 h reperfusion followed by cerebral ischemia 10 min; And cerebral ischemia - reperfusion （without preconditioning） group （IR）. Observations were carried out in each subgroup 1 d、3d, 5d and 7d （each subgroup n =6）, the apoptosis neurons in hippocampus CA1 region was counted. Results： The number of apoptosis neurons in hippocampus CA1 region were much less in group IP than in group IR （ P 〈 0.01 ）. Conclusion： Ischemic preconditioning can significantly enhance the tolerance of the following ischernia, The mechanism of protection may be that ischemic preconditioning can prevent neurons from apoptosis induced by ischemia.
出处
《泰山医学院学报》
CAS
2005年第4期312-315,共4页
Journal of Taishan Medical College
关键词
海马
缺血预处理
细胞凋亡
hippocampus
ischemic preconditioning
apoptosis
