摘要
本实验用测定培养液中葡萄糖醛酸的方法,作为培养软骨细胞生长情况的指标,以观察软骨细胞的代谢情况,了解不同种类和不同含量血清对培养软骨细胞的影响.我们用传代兔软骨细胞于不同含量小牛血清和人血清的培养液中培养,定期镜下观察并收集培养液,测定其葡萄糖醛酸含量.结果表明5%至15%牛血清或人血清培养一代兔软骨细胞均可生长;测定培养液內葡萄糖醛酸量作为反映软骨细胞生长的检测指标是可行的。
In this paper, We present the method of using glucuronate as a parameter to determine the growth of cartilage cell in the culture media. Using this method, we observed the metabolic condition of cartilage cell and knew the effect of the serum with the various types and different concentration to the growth of cartilage cell. The. experimental method was used for the first passage chondrocyte of rabbit. We put the cartilage cell in the culture media containing different concentration of serum from calf and human; then, observed the growth of cartilage cell under the microscope, collected the medium in given time; and determined the amount of glucuronate in the medium. Our results indicated that the first passage chondrocyte of rabbit can grow in the medium containing serum of calf and human in 5%～15% Also, the study shows that it is possible to determine the amount of glucuronate in the medium as a parameter to respond the growth of the cartilage cells.
出处
《西安医科大学学报》
CSCD
1990年第1期44-47,共4页
Journal of Xi'an Medical University(Chinese)
