摘要
目的观察大鼠肾脏创伤早期肾脏和下丘脑ADM表达的变化及外源性ADM对二者表达的影响。方法将健康成年普通级Wistar大鼠随机分为对照组、创伤组(32只);预防组(32只)和治疗组(32只)4组,后3组均采用自由落体打击仪直接打击大鼠脊肋区制作创伤模型,两给药组分别于创伤前后10min腹腔注射ADM(0.1nmol/kg)。平均分为四批于创伤后1、6、12、24h采用快速心脏采血法处死。迅速解剖动物提取肾脏及下丘脑标本,中性福尔马林固定,免疫组织化学染色,观察ADM在肾脏及下丘脑组织中的表达,并进行统计学分析。结果肾脏ADM阳性表达:预防组于伤后1h阳性表达,其阳性结果随时间呈上升趋势,与创伤组及对照组相比,差异有统计学意义(P<0.05)。治疗组于伤后1h、6h强阳性表达,并随时间呈下降趋势,至伤后24h恢复正常水平,差异有统计学意义(P<0.05)。创伤组ADM呈低阳性表达,以1h及12h为著,24h基本恢复正常,差异有统计学意义(P<0.05)。下丘脑ADM阳性表达:治疗组阳性表达趋势基本同肾脏ADM阳性表达趋势,与创伤组及对照组相比,差异有统计学意义(P<0.05)。预防组于6h及24h呈强阳性表达;12h阳性表达结果明显降低且达最低峰,差异有统计学意义(P<0.05)。创伤组于6h阳性表达结果明显降低,与对照组相比,差异有统计学意义(P<0.05)。结论下丘脑ADM对肾脏ADM的表达有一定的上调作用,ADM通过局部及全身的正负反馈调节以维持机体内环境的相对稳定和正常生理活动。
Objective To observe the effects of exogenous adrenomedullin （ADM） on endogenous expression of ADM in the kidney and hypothalamus of rats early after mechanical renal trauma. Methods Adult Wistar rats were randomized into 4 groups （n=32）, namely the control group, renal impact trauma group, preventive ADM injection group, and therapeutic ADM injection group. In the latter two groups, ADM （0.1 nmol/kg） was administrated by intrapertoneal injection 10 min before and 10 min after renal trauma. The rats were executed at 1, 6, 12, and 24 h after the trauma to examine the expression of ADM in the kidney and hypothalamus. Results In preventive ADM injection group, the renal expression of ADM increased significantly at 1 h after the trauma （P〈0.05） and tended to further increase with time till 24 h when its expression recovered the normal level. In the therapeutic ADM injection group, strong renal ADM positivity was found at 1 and 6 h after the injury （P〈0.05） followed by gradual decrease till recovering the normal level at 24 h. Low renal ADM expression was detected, which was the strongest at 1 and 12 h （P〈0.05） and became normal at 24 h. The time course of ADM expression in the hypothalamus was similar to that in the kidney in the therapeutic ADM injection group, and in the preventive injection group, the strongest ADM expression in the hypothalamus occurred at 6 and 24 h, and the lowest expression occurred at 12 h （P〈0.05）. The trauma group showed significantly decreased ADM expression in the hypothalamus compared with the control group （P〈0.05）. Conclusion The hypothalamic ADM expression can upregulate renal ADM expression. ADM maintains the relative stability of the internal environment and physiological activity by local and systemic positive and negative feedback mechanisms
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2011年第6期1081-1085,共5页
Journal of Southern Medical University
基金
兰州大学医学科研基金(V200805)
