摘要
目的观察骨髓基质细胞在多孔状的人工骨块上三维立体培养后的生长情况及其复合植入体内后的成骨能力。方法利用组织工程学方法，将骨髓基质细胞种植于羟基磷灰石人工骨块上，立体培养２周，用扫描电镜观察细胞在体外的生长情况；将上述细胞人工骨复合体自体异位植入体内，取材观察其植入体内后的成骨情况。结果细胞在人工骨块上能立体培养成活，细胞多生长于周边的孔隙表面，尤其以贴近培养瓶底的那一边较多，骨块的中心部位未见细胞生长。自体异位植入后４周，大多数的人工骨中可见新骨形成，新形成的骨多紧贴于人工骨的表面，并可见活性的成骨细胞、骨样组织及类似于正常骨组织中骨髓腔样的腔隙组织。结论骨髓基质细胞可用作骨组织工程学的种子细胞，多孔状的羟基磷灰石是可利用的细胞载体。
Objective To assess the ability of bone graft substitutes to support the growth of cells in vitro and new bone formation in vivo. Methods The methods of tissue engineering were employed,the bone marrow derived stromal cells were seeded onto blocks of hydroxypatite(HA), cultured for two weeks,and implanted intermuscularly into the rabbit back muscle,scanning electron microscopy and undecalcified bone tissue sections were used to evaluate the the growth of cells in vitro and new bone formation in vivo. Results The stromal cells were attached to and grew upon the surface of the periphery HA pores, ecpecially in the pores adjacent to the bottom of culture flasks. No cell growth was found on the surface of the central pores. At four weeks after implantation, new bone formation was observed in most HA blocks, almost all of the new bones were lamellar bone and deposited directly on the surface of the HA pore. Active osteoblast and osteoid were found on the surface of the newly formed bone, invasion of bone marrow was observed in some newly formed bone. Conclusion Bone marrow stromal cells are patentially the source of seeded cells for use in bone tissue engineering, and the HA may be considered as a suitable scaffold for these cells. The findings may be useful for detailed reseraches on seeded cells,scaffold and implanting fashion of bone tissue engineering.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第7期395-398,I027,共5页
Chinese Journal of Surgery
基金
国家自然科学基金
