摘要
目的研究牛磺酸对锰致大鼠海马线粒体超微结构改变及氧化损伤的保护作用。方法 W istar大鼠80只随机分为对照组、染锰组、牛磺酸预防组、牛磺酸治疗组,连续染毒12周后制备海马线粒体,电镜扫描超微超微结构变化,测定线粒体活性氧簇(ROS)变化;体外制备成年健康大鼠的海马线粒体并与锰、牛磺酸孵育,观察ROS变化。结果染锰致线粒体空泡化、嵴消失,牛磺酸可改善线粒体的超微结构损伤;体内染锰后染锰组海马线粒体ROS为(170.1±6.1),明显低于对照组(182.2±5.5)和牛磺酸预防组(207.0±6.0),高于牛磺酸治疗组(150.2±3.8),差异均有统计学意义(t=3.63,t=11.61,t=6.31,P<0.05);线粒体体外染锰后染锰组ROS为(2 139.2±24.9),明显高于对照组(650.7±12.0)、牛磺酸预防组(1 734.5±20.7)和牛磺酸治疗组(1 477.3±14.8),差异均有统计学意义(t=137.23,t=37.31,t=61.01,P<0.05)。结论牛磺酸可改善染锰损伤的大鼠海马线粒体超微结构;体外实验结果表明,用牛磺酸预防和治疗染锰大鼠均能明显降低其ROS。
Objective To investigate the effects of taurine on hippocampus mitochondrial ultrastracture changes and oxidative damage induced by MnCl2.4H2O.Methods Eighty Wistar rats were divided randomly into 4 groups:control group,MnCl2.4H2O-treatment group,taurine-treatment group,and taurine-prevention group.Hippocampus mitochondria was isolated and prepared for electroscopic scanning and reactive oxygen species(ROS) detection.Hippocampus mitochondria from healthy rats was also separated and incubated with MnCl2.4H2O and taurine in vitro for determination of ROS.Results The dissolve of mitochondrial membrane and cristae and the degeneration of mitochondrial vacuolar were observed in MnCl2 group.Both taurine treatment and prevention could improve mitochondrial ultrastracture damage induced by MnCl2.ROS in MnCl2 group(170.3±6.1) was significantly lower than that of in the control(182.2±5.5) and taurine-prenvention group(207.0±6.0)(t=3.63,P<0.05;t=11.61,P<0.05).While ROS in taurine treatment(150.2±3.8) group was the lowest and was different from that of MnCl2 administration(t=6.31,P<0.05).In vitro,compared with that of the control(650.7±12.0),ROS was significantly higher in MnCl2 treatment group(2139.2±24.9,t=137.23;P<0.05).Taurine-prevention(1734.5±20.7) and taurine-treatment(1477.3±14.8) could markedly down-regulrate ROS(t=37.31,P<0.05;t=61.01,P<0.05).Conclusion MnCl2 treatment could induce ultrastracture morphologic damage of hippocampns mitochonhria in vivo and taurine-prevention and taurine-treaement could evidently down-regulrate the ROS induced by MnCl2.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第7期889-891,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30660156)
广西研究生教育创新计划(2009105981004D24)
关键词
牛磺酸
锰
线粒体
超微结构
氧化损伤
taurine
manganese
mitochondria
ultrasctrature
oxidative damage
