摘要
目的探讨锰染毒大鼠空间学习记忆变化的规律。方法50只雄性大鼠适应性喂养1周后,随机分为5组,即生理盐水组(A组),Mn2+2.5,5,10,20 mg/kg(B、C、D、E组)。腹腔注射生理盐水或MnCl2溶液0.5 mL/d,共30 d。染毒结束后,采用Morris水迷宫试验测定大鼠潜伏期、总路程和穿台次数的变化。结果经多组比较的单因素方差分析,潜伏期、总路程、穿台次数各组均数间差异有统计学意义(F=7.813,P=0.000;F=8.190,P=0.000;F=3.461,P=0.000);与A组比较,B、C、D和E组潜伏期延长,差异有统计学意义(P<0.05);与B组比较,D和E组潜伏期延长,差异有统计学意义(P<0.05)。与A组比较,C、D和E组总路程延长,差异有统计学意义(P<0.01);与B组比较,C、D和E组总路程延长,差异有统计学意义(P<0.01)。与A组比较,B、C、D、E 4组穿台次数减少,差异有统计学意义(P<0.05)。结论锰染毒可以引起大鼠空间学习记忆障碍。
Objective To study the effect of manganese（Mn） on long-term spatial learning and memory capability in rats.Methods Fifty male Sprague Dawley rats were randomly assigned to five groups：normal saline（Group A）,Mn2＋ 2.5mg/kg（Group B）,Mn2＋ 5mg/kg（Group C）,Mn2＋ 10mg/kg（Group D）,and Mn2＋ 20mg/kg（Group E）.Each experimental group consisted of 10 animals.Morris water maze was used to examine the mean latency,pathlength,and the number of platform area crossing of the rats.Results In Morris water maze test,significant increases were found in mean latency,pathlength,and the number of platform area crossing in the groups（F=7.813,P=0.000;F=8.190,P=0.000;F=3.461,P=0.000）.Significant increases were found in the mean latency of groups B,C,D and E compared with that of group A（P0.05）.There was an obvious increase in the pathlength in the groups C,D and E compared with group A（P0.01）.Compared with group B,there were significant increases in the mean latency in group D and E（P0.05） and in the number of platform area crossing in the groups C,D and E（P0.01）.Conclusion Manganese can affect spatial learning and memory ability in rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第3期381-382,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30700657)
北京市优秀人才培养资助计划(20071D0501800250)
