摘要
目的探讨电磁辐射对Th1/Th2细胞因子平衡的影响。方法以平均功率密度5 mW/cm2的微波辐照昆明小鼠,30 m in/d,连续30 d,采用逆转录聚合酶链式反应(RT-PCR)、ELISA法检测微波辐照后干扰素(INF-γ)、白细胞介素-4(IL-4)表达量的变化,分析微波辐照对Th1/Th2平衡的影响。结果 5 mW/cm2微波累积辐照导致INF-γ基因及蛋白表达水平增高,小鼠血清中INF-γ含量在微波累积辐照第8 d为(430.29±41.95)pg/mL,达到峰值,增幅为27.84%(P<0.05);微波累积辐照使IL-4的表达有降低的趋势,但与正常组比较,差异无统计学意义(P>0.05)。结论平均功率密度为5 mW/cm2的微波累积辐照导致昆明种小鼠Th1/Th2平衡向Th1方向偏移。
Objective To observe the influence of electromagnetic irradiation on Th1/Th2 cytokine balance.Methods The mice were continuously exposed to 5 mW/cm2 electromagnetic wave 30 min/day for 30 days.The expression levels of interferon-gamma（INF-γ）and interleukin-4（IL-4） were detected with reverse transcription polymerase chain reaction（RT-PCR） and enzyme-linked immunosorbent assay（ELISA）.The excursion of Th1/Th2 cytokine balance induced by electromagnetic irradiation was analysed.Results The INF-γ expression level was increased by 5 mW/cm2 electromagnetic irradiation.At the 8th day of the electromagnetic irradiation,the INF-γ level in murine serum was increased by 27.84% compared with that of the sham group（P0.05）.The IL-4 expression level showed a decrease tendency after the electromagnetic irradiation but the decrease was not significant compared with that of the sham（P0.05）.Conclusion The 5 mW/cm2 electromagnetic irradiation can lead the deflection of Th1/Th2 cytokine balance to Th1.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第7期887-888,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30972432)
