摘要
目的建立同时测定消毒产品中甲硝唑、盐酸金霉素、土霉素二水合物、盐酸米诺环素、红霉素及盐酸四环素的高效液相色谱-串联质谱(LC-MS/MS)检测方法。方法样品经甲醇超声提取,0.45μm聚四氟乙烯膜过滤后,选择WatersSymmetryC18(2.1mm×150mm,3.5μm)为分析柱,5mmol/L乙酸铵缓冲溶液、甲醇和乙腈为流动相,用LC-MS/MS正离子扫描多反应监测模式检测。结果上述6种抗生素在0～2000ng/ml范围内线性关系良好,相关系数均大于0.998,回收率为74.7%～114%,相对标准偏差为1.6%～20.2%。该法成功用于115件皮肤、黏膜使用的膏霜剂型抗(抑)菌制剂消毒产品中抗生素残留量的检测。结论该法操作简单,灵敏度高,选择性强,可用于膏霜剂型消毒产品中抗生素残留量的测定。
Objective To develop a method for determination of the Metronidazole,Chlortetracycline hydrochloride,Oxytetracycline dihydrate,Minocycline hydrochloride,Erythromycin and Tetracycline hydrochloride in disinfection products by high performance liquid chromatography tandem mass spectrometry（LC-MS/MS）.Methods Samples were extracted by methanol and filtered through 0.45μm PTFE membrane filter,then analyzed by LC-MS/MS using Waters SymmetryC18（2.1mm×150mm,3.5μm）column in positive ion scan mode.The mobile phase was 5mmol/L ammonium acetate,methanol and acetonitrile.Results The linear range was 0-2000ng/ml and the correlation coefficients were more than 0.998,the average recoveries ranged from 74.7% to 114% with the relative standard deviations between 1.6%-20.2%.The method was successfully used to detect the content of antibiotics in 115 disinfection products.Conclusion The method is simple,sensitive,selective and suitable for the analysis of residual content of antibiotics in cream formulations of disinfection products.
出处
《卫生研究》
CAS
CSCD
北大核心
2012年第2期282-286,共5页
Journal of Hygiene Research
