摘要
在克隆花粉发育相关基因BcMF13时,用RACE技术进行3'端扩增,得到了两个不同长度的3'转录本。与EST库比对,发现与其相似性高的序列都来源于十字花科芸薹属、萝卜属等植物的生殖器官,并且在这些近源属中也出现不同转录本。这些不同转录本的存在说明BcMF13存在剪切异构体,调控过程灵活复杂,反映出该基因具有重要功能。同时,对BcMF13基因的RT-PCR表达研究表明,它只在可育株系中表达,在不育株系中不表达,且主要集中在大花蕾、雄蕊中表达。分析BcMF13推导的蛋白结构,发现其编码的蛋白包含多个生物活性位点。以上结果均表明BcMF13与花粉育性相关,多个剪切异构体的发现说明BcMF13在执行生理功能时较为活跃。
Two different lengths of 3＇ transcripts were got in cloning the pollen development gene BcMF13, using RACE technology. Compared with the EST database and found that their high sequence similarity are derived fiom cruciferous Brassica, Raphanus, plants＇ reproductive organs, and different transcripts were also found in these sources. The existence of these different transcripts exist that BcMF13 shear isomers, flexible and complex regulation of the process, reflecting the important function of the gene. Expression analysis of RT-PCR have shown that BcMFI3 only expressed in fertile lines, no in sterile lines, and mainly concentrated in the large flower buds and stamen. Analysis of the deduced protein structure of BcMF13 found that the proteins encoded multiple biological active sites. These results show that BcMF13 was late expression gene on pollen fertility. BcMF13 was active in the implementation of physiological ihnction.
出处
《植物分类与资源学报》
CAS
CSCD
北大核心
2011年第3期330-334,共5页
Plant Diversity
基金
国家自然科学基金"多肽激素类基因RALF在油菜花粉发育中的功能研究"(31000132)
安徽省高校青年教师资助项目"植物肽类激素RALF基因的分离和遗传转化"(2008jq1121)
